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Literature summary for 6.1.1.26 extracted from

  • Herring, S.; Ambrogelly, A.; Polycarpo, C.R.; Soll, D.
    Recognition of pyrrolysine tRNA by the Desulfitobacterium hafniense pyrrolysyl-tRNA synthetase (2007), Nucleic Acids Res., 35, 1270-1278.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
gene pylS, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Desulfitobacterium hafniense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of pyrrolysylation of several mutant tRNAPyls, overview Desulfitobacterium hafniense
0.0029
-
 tRNAPyl pH 7.2, 37°C, wild-type tRNAPyl Desulfitobacterium hafniense

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Desulfitobacterium hafniense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl Desulfitobacterium hafniense pyrrolysyl-tRNA synthetase PylRS attaches L-pyrrolysine to its cognate tRNA, the special amber suppressor tRNAPyl, encoded by gene pylT AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Organism

Organism UniProt Comment Textmining
Desulfitobacterium hafniense
-
 gene pylS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Desulfitobacterium hafniense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl pyrrolysyl-tRNA synthetase PylRS attaches L-pyrrolysine to its cognate tRNA, the special amber suppressor tRNAPyl, encoded by gene pylT Desulfitobacterium hafniense AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
ATP + L-pyrrolysine + tRNAPyl PylRS binds tRNA predominantly along the phosphate backbone of the T-loop, the D-stem and the acceptor stem, while no significant contacts with the anticodon arm occur, the tRNAPyl anticodon is not important for recognition by bacterial PylRS, overview Desulfitobacterium hafniense AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
ATP + N-epsilon-cyclopentyloxycarbonyl-L-lysine + tRNAPyl substrate specificity, ability of 24 mutant tRNA species to be aminoacylated by the enzyme with the pyrrolysine analog N-epsilon-cyclopentyloxycarbonyl-L-lysine, overview, the discriminator base G73 and the first base pair G1-C72 in the acceptor stem are major identity elements Desulfitobacterium hafniense AMP + diphosphate + N-epsilon-cyclopentyloxycarbonyl-L-lysyl-tRNAPyl
-
 ?
additional information enzyme activity with wild-type and different mutant tRNAPyls, overview, 3'-labeled tRNAPyl footprinting with S1 and T1 nuclease digestions, overview Desulfitobacterium hafniense ?
-
 ?

Synonyms

Synonyms Comment Organism
PylRS
-
 Desulfitobacterium hafniense
pyrrolysyl-tRNA synthetase
-
 Desulfitobacterium hafniense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Desulfitobacterium hafniense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 tRNAPyl pH 7.2, 37°C, wild-type tRNAPyl Desulfitobacterium hafniense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Desulfitobacterium hafniense

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Desulfitobacterium hafniense