Any feedback?
Literature summary for 6.1.1.26 extracted from
- An aminoacyl-tRNA synthetase that specifically activates pyrrolysine (2004), Proc. Natl. Acad. Sci. USA, 101, 12450-12454.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pylS, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Methanosarcina barkeri |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Methanosarcina barkeri |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-pyrrolysine + tRNAPyl | Methanosarcina barkeri | - |
AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
| ATP + L-pyrrolysine + tRNAPyl | Methanosarcina barkeri Fusaro / DSM 804 | - |
AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
| additional information | Methanosarcina barkeri | Methanosarcina cells have two pathways for acylating the suppressor tRNAPyl to ensure efficient translation of the in-frame UAG codon in case of pyrrolysine deficiency and safeguard the biosynthesis of the proteins whose genes contain this special codon, L-pyrrolysine is found in the Methanosarcina barkeri monomethylamine methyltransferase protein in a position that is encoded by an in-frame UAG stop codon in the mRNA, overview | ? | - |
? | |
| additional information | Methanosarcina barkeri Fusaro / DSM 804 | Methanosarcina cells have two pathways for acylating the suppressor tRNAPyl to ensure efficient translation of the in-frame UAG codon in case of pyrrolysine deficiency and safeguard the biosynthesis of the proteins whose genes contain this special codon, L-pyrrolysine is found in the Methanosarcina barkeri monomethylamine methyltransferase protein in a position that is encoded by an in-frame UAG stop codon in the mRNA, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
gene pylS | - |
| Methanosarcina barkeri Fusaro / DSM 804 | - |
gene pylS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-pyrrolysine + tRNAPyl | - |
Methanosarcina barkeri | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
| ATP + L-pyrrolysine + tRNAPyl | the enzyme uses a special amber suppressor tRNA, tRNAPyl, that presumably recognizes this UAG codon, and does not accept L-lysine or tRNALys as substrates, direct transfer of L-pyrroslysine to the UAG codon of tRNAPyl, overview | Methanosarcina barkeri | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
| ATP + L-pyrrolysine + tRNAPyl | - |
Methanosarcina barkeri Fusaro / DSM 804 | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
| ATP + L-pyrrolysine + tRNAPyl | the enzyme uses a special amber suppressor tRNA, tRNAPyl, that presumably recognizes this UAG codon, and does not accept L-lysine or tRNALys as substrates, direct transfer of L-pyrroslysine to the UAG codon of tRNAPyl, overview | Methanosarcina barkeri Fusaro / DSM 804 | AMP + diphosphate + L-pyrrolysyl-tRNAPyl | - |
? | |
| additional information | Methanosarcina cells have two pathways for acylating the suppressor tRNAPyl to ensure efficient translation of the in-frame UAG codon in case of pyrrolysine deficiency and safeguard the biosynthesis of the proteins whose genes contain this special codon, L-pyrrolysine is found in the Methanosarcina barkeri monomethylamine methyltransferase protein in a position that is encoded by an in-frame UAG stop codon in the mRNA, overview | Methanosarcina barkeri | ? | - |
? | |
| additional information | structure of endogenous tRNAPyl, overview | Methanosarcina barkeri | ? | - |
? | |
| additional information | Methanosarcina cells have two pathways for acylating the suppressor tRNAPyl to ensure efficient translation of the in-frame UAG codon in case of pyrrolysine deficiency and safeguard the biosynthesis of the proteins whose genes contain this special codon, L-pyrrolysine is found in the Methanosarcina barkeri monomethylamine methyltransferase protein in a position that is encoded by an in-frame UAG stop codon in the mRNA, overview | Methanosarcina barkeri Fusaro / DSM 804 | ? | - |
? | |
| additional information | structure of endogenous tRNAPyl, overview | Methanosarcina barkeri Fusaro / DSM 804 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | cf. EC 6.1.1.25 | Methanosarcina barkeri |
| pyrrolysyl-tRNA synthetase | - |
Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Methanosarcina barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Methanosarcina barkeri | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
