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Literature summary for 6.1.1.26 extracted from

  • Wang, Y.S.; Fang, X.; Wallace, A.L.; Wu, B.; Liu, W.R.
    A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum (2012), J. Am. Chem. Soc., 134, 2950-2953.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
N346A/C348A the mutant displays specific recognition toward non-canonical amino acids and has a broad substrate spectrum with specific recognition of L-phenylalanine Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl Escherichia coli
-
AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl
-
Escherichia coli AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
?

Synonyms

Synonyms Comment Organism
PylRS
-
Escherichia coli
pyrrolysyl-tRNA synthetase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli