Literature summary for 6.1.1.26 extracted from

Wang, Y.S.; Russell, W.K.; Wang, Z.; Wan, W.; Dodd, L.E.; Pai, P.J.; Russell, D.H.; Liu, W.R.
The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives (2011), Mol. Biosyst., 7, 714-717.

Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant PylRS in Escherichia coli	Methanosarcina mazei

Protein Variants

Protein Variants	Comment	Organism
additional information	evolving a PylRS mutant that specifically acylates tRNAPylCUA with L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine by standard positive and negative selections of the pRS1 plasmid library in Escherichia coli. The pRS1 plasmid library contains the Methanosarcina mazei PylRS gene with randomization at six active-site residues, L305, Y306, L309, N346, C348, and W417, method, overview	Methanosarcina mazei
additional information	usage of evolved pyrrolysyl-tRNA synthetase-tRNAPylCUA pairs for genetic incorporation of L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine into proteins at amber mutation sites in Escherichia coli	Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	-	-	-
Methanosarcina mazei	-	-	-

Synonyms

Synonyms	Comment	Organism
pyrrolysyl-tRNA synthetase	-	Methanocaldococcus jannaschii
pyrrolysyl-tRNA synthetase	-	Methanosarcina mazei

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Release 2023.1 (January 2023)