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Literature summary for 6.1.1.26 extracted from

  • Yanagisawa, T.; Ishii, R.; Fukunaga, R.; Kobayashi, T.; Sakamoto, K.; Yokoyama, S.
    Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modification (2008), Chem. Biol., 15, 1187-1197.
    View publication on PubMed
Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina mazei

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of a catalytic fragment of the enzyme complexed with N3-(tert-butyloxycarbonyl)-L-lysine and an ATP analog and with Nepsilon-allyloxycarbonyl-L-lysine reveals that the enzyme requires an Nepsilon-carbonyl group bearing a substituent with a certain size Methanosarcina mazei

Protein Variants

Protein Variants Comment Organism
Y306/Y384F together with tRNAPyl the mutant enzyme provides a good yield of the in vivo amber-suppression product containing Nepsilon-benzyloxycarbonyl-L-lysine Methanosarcina mazei
Y306A mutation of PylRS drastically increases the in vitro aminoacylation activity for Nepsilon-benzyloxycarbonyl-L-lysine Methanosarcina mazei
Y384F the mutation increases the aminoacylation activity of the enzyme regardless of the amino acid substrate Methanosarcina mazei

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei Q8PWY1
-
-
Methanosarcina mazei DSM 3647 Q8PWY1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Nepsilon-(N-methylanthraniloyl)-L-lysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + Nepsilon-(N-methylanthraniloyl)-L-lysyl-tRNAPyl
-
 ?
ATP + Nepsilon-(N-methylanthraniloyl)-L-lysine + tRNAPyl
-
 Methanosarcina mazei DSM 3647 AMP + diphosphate + Nepsilon-(N-methylanthraniloyl)-L-lysyl-tRNAPyl
-
 ?
ATP + Nepsilon-(tert-butyloxycarbonyl)-L-lysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + Nepsilon-(tert-butyloxycarbonyl)-L-lysyl-tRNAPyl
-
 ?
ATP + Nepsilon-(tert-butyloxycarbonyl)-L-lysine + tRNAPyl
-
 Methanosarcina mazei DSM 3647 AMP + diphosphate + Nepsilon-(tert-butyloxycarbonyl)-L-lysyl-tRNAPyl
-
 ?
ATP + Nepsilon-allyloxycarbonyl-L-lysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + Nepsilon-allyloxycarbonyl-L-lysyl-tRNAPyl
-
 ?
ATP + Nepsilon-allyloxycarbonyl-L-lysine + tRNAPyl
-
 Methanosarcina mazei DSM 3647 AMP + diphosphate + Nepsilon-allyloxycarbonyl-L-lysyl-tRNAPyl
-
 ?
ATP + Nepsilon-benzyloxycarbonyl-L-lysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + Nepsilon-benzyloxycarbonyl-L-lysine-tRNAPyl
-
 ?
ATP + Nepsilon-benzyloxycarbonyl-L-lysine + tRNAPyl
-
 Methanosarcina mazei DSM 3647 AMP + diphosphate + Nepsilon-benzyloxycarbonyl-L-lysine-tRNAPyl
-
 ?
ATP + Nepsilon-nicotinoyl-L-lysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + Nepsilon-nicotinoyl-L-lysyl-tRNAPyl
-
 ?
additional information Nepsilon-(p-azidobenzoyl)-L-lysine, Nepsilon-biotinyl-L-lysine, and Nepsilon-(9-fluorenylmethoxycarbonyl)-L-lysine, which have even larger substituents at the N3-carbonyl group, are not detectably esterified to tRNAPyl. The enzyme exhibits no ligation activity for lysine derivatives without the Nepsilon-carbonyl group, such as Nepsilon-methyl-L-lysine, Nepsilon-dimethyl-L-lysine, Nepsilon-trimethyl-L-lysine, Nepsilon-isopropyl-L-lysine, Nepsilon-dansyl-L-lysine, Nepsilon-(o,p-dinitrophenyl)-L-lysine, Nepsilon-(p-toluenesulfonyl)-L-lysine, and Nepsilon-(DL-2-amino-2-carboxyethyl)-L-lysine, regardless of the size of the Nepsilon-substituent Methanosarcina mazei ?
-
 ?
additional information Nepsilon-(p-azidobenzoyl)-L-lysine, Nepsilon-biotinyl-L-lysine, and Nepsilon-(9-fluorenylmethoxycarbonyl)-L-lysine, which have even larger substituents at the N3-carbonyl group, are not detectably esterified to tRNAPyl. The enzyme exhibits no ligation activity for lysine derivatives without the Nepsilon-carbonyl group, such as Nepsilon-methyl-L-lysine, Nepsilon-dimethyl-L-lysine, Nepsilon-trimethyl-L-lysine, Nepsilon-isopropyl-L-lysine, Nepsilon-dansyl-L-lysine, Nepsilon-(o,p-dinitrophenyl)-L-lysine, Nepsilon-(p-toluenesulfonyl)-L-lysine, and Nepsilon-(DL-2-amino-2-carboxyethyl)-L-lysine, regardless of the size of the Nepsilon-substituent Methanosarcina mazei DSM 3647 ?
-
 ?

Synonyms

Synonyms Comment Organism
PylRS
-
 Methanosarcina mazei
pyrrolysyl-tRNA synthetase
-
 Methanosarcina mazei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina mazei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Methanosarcina mazei