6.1.1.19: arginine-tRNA ligase
This is an abbreviated version!
For detailed information about arginine-tRNA ligase, go to the full flat file.
Word Map on EC 6.1.1.19
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6.1.1.19
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aminoacyl-trna
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synthetases
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aminoacylation
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arginylation
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anticodon
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pontocerebellar
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aarss
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isoacceptors
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glnrs
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atp-ppi
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multisynthetase
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lysyl-trna
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trnaasp
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l-canavanine
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glutaminyl-trna
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aspartyl-trna
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isoleucyl
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phenylalanyl-trna
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glutamyl-prolyl-trna
- 6.1.1.19
- aminoacyl-trna
- synthetases
- aminoacylation
-
arginylation
-
anticodon
-
pontocerebellar
-
aarss
-
isoacceptors
- glnrs
-
atp-ppi
-
multisynthetase
- lysyl-trna
- trnaasp
- l-canavanine
- glutaminyl-trna
- aspartyl-trna
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isoleucyl
- phenylalanyl-trna
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glutamyl-prolyl-trna
Reaction
Synonyms
Arg-tRNA synthetase, Arginine translase, Arginine--tRNA ligase, Arginine-tRNA synthetase, Arginyl transfer ribonucleic acid synthetase, Arginyl-transfer RNA synthetase, Arginyl-tRNA synthetase, arginyl–tRNA synthetase, ArgRS, ArgS2, MtArgRS, RARS, RARS2, RRS, Synthetase, arginyl-transfer ribonucleate
ECTree
6.1.1.19 arginine-tRNA ligaseAdvanced search results
results (
results (KM Value
KM Value on EC 6.1.1.19 - arginine-tRNA ligase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
additional information
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
0.207
ATP
pH 7.5, 30°C, recombinant HIS-/thioredoxin-tagged enzyme
0.501
ATP
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pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin
0.529
ATP
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pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin
0.001
L-arginine
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ATP-diphosphate exchange reaction, pH 8, 55°C
0.0022
L-arginine
assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid
0.003
L-arginine
assay method, aminoacylation of [32P]-labelled transcript tRNA
0.0065
L-arginine
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pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin
0.0074
L-arginine
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ATP-diphosphate exchange reaction, pH 8, 37°C
0.0078
L-arginine
assay method, aminoacylation of [32P]-labelled transcript tRNA
0.0078
L-arginine
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pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin
0.0137
L-arginine
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aminoacylation reaction, 4-fluorotryptophan-labeled enzyme
0.0164
L-arginine
pH 7.5, 30°C, recombinant HIS-/thioredoxin-tagged enzyme
0.0196
L-arginine
assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid
0.0453
L-canavanine
assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid
0.0454
L-canavanine
assay method, aminoacylation of [32P]-labelled transcript tRNA
1.32
L-canavanine
assay method, aminoacylation of [32P]-labelled transcript tRNA
0.0002
tRNAArg
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substrate from E. coli, , ATP-diphosphate exchange, pH 6.0
0.00032
tRNAArg
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25°C, pH 7.5, native tRNAArg from beef liver, wild type enzyme
0.00039
tRNAArg
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25°C, pH 7.5, native tRNAArg from beef liver, mutant enzyme DELTA1-73
0.0036
tRNAArg
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substrate from E. coli, , ATP-diphosphate exchange, pH 7.4
0.00477
tRNAArg
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pH 7.5, 50°C, recombinant MtArgRS in presence of recombinant MtSerRS
0.0081
tRNAArg
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pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg
0.0096
tRNAArg
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pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg
0.0098
tRNAArg
adenine at position 20 of the tRNAArg is required for activity, pH 7.5, 65°C
0.45
tRNAArg
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25°C, pH 7.5, tRNAArg from Saccharomyces cerevisiae, aminoacylation
0.00184
tRNAArg ACG
pH 7.5, 30°C, recombinant HIS-/thioredoxin-tagged enzyme
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0.00029
tRNAArg(ACG)
tRNA from Escherichia coli, pH and temperature not specified in the publication
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0.0014
tRNAArg(ACG)
tRNA from Glycine max, pH and temperature not specified in the publication
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0.0017
tRNAArg(ACG)
tRNA from Glycine max, pH and temperature not specified in the publication
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0.0022
tRNAArg(ACG)
tRNA from Escherichia coli, pH and temperature not specified in the publication
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0.0024
tRNAArg(ACG)
tRNA from Escherichia coli, pH and temperature not specified in the publication
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0.0041
tRNAArg(ACG)
tRNA from Glycine max. pH and temperature not specified in the publication
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0.2
tubercidin 5'-triphosphate
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formycin 5'-triphosphate, 2'-deoxyadenosine 5'-triphosphate, 3'-O-methyladenosine 5'-triphosphate
0.4
tubercidin 5'-triphosphate
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3'-methoxyadenosine 5'-triphosphate, , NTP-diphosphate exchange
additional information
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide
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Km values for different tRNAArg variants
additional information
additional information
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Km values for aminoacylation of various amino acids with tRNAArg-C-C-A and tRNAArg-C-C-A(3'NH2)
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additional information
additional information
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effect on the Km of several variations of the tRNAArg sequence
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additional information
additional information
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Km values for ATP and L-arginine for 26 mutants
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additional information
additional information
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Km values for ATP and L-arginine for several mutants and revertants
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additional information
additional information
Km values for the wild type and mutant enzymes using tRNAArg with different nucleotides at position 20
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additional information
additional information
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Km values for the wild type and mutant enzymes using tRNAArg with different nucleotides at position 20
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additional information
additional information
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addition of MtArgRS to MtSerRS leads to an almost 4fold increase in the catalytic efficiency of serine attachment to tRNA, also under conditions of elevated temperature and osmolarity, but has no effect on the activity of MtArgRS, steady-state kinetic analyses, overview
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additional information
additional information
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Km values for tRNAArg, arginine, and ATP in the presence of hemin are not altered, but kcat values dramatically decrease compared with those in the absence of hemin, kinetic analysis, overview
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