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Literature summary for 6.1.1.19 extracted from

  • Gangloff, J.; Schutz, A.; Dirheimer, G.
    Arginyl-tRNA synthetase from baker's yeast. Purification and some properties (1976), Eur. J. Biochem., 65, 177-182.
    View publication on PubMed
Search for more literature for 6.1.1.19

General Stability

General Stability Organism
not affected by four freeze-thaw cycles Saccharomyces pastorianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00028
-
 tRNAArgIII aminoacylation Saccharomyces pastorianus
0.0015
-
 Arg aminoacylation Saccharomyces pastorianus
0.0025
-
 tRNAArg Arg, , ATP-diphosphate exchange Saccharomyces pastorianus
0.05
-
 diphosphate ATP-diphosphate exchange Saccharomyces pastorianus
0.3
-
 ATP aminoacylation Saccharomyces pastorianus
1.4
-
 ATP ATP-diphosphate exchange Saccharomyces pastorianus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2, optimal concentration in aminoacylation: 7 mM , when ATP concentration is 10 mM Saccharomyces pastorianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 gel filtration Saccharomyces pastorianus
70000 73000 sucrose density gradient centrifugation Saccharomyces pastorianus
74000
-
 1 * 74000, SDS-PAGE Saccharomyces pastorianus

Organism

Organism UniProt Comment Textmining
Saccharomyces pastorianus
-
-
-
Saccharomyces pastorianus 836
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large-scale Saccharomyces pastorianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-arginine + tRNAArg
-
 Saccharomyces pastorianus AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
ATP + L-arginine + tRNAArg
-
 Saccharomyces pastorianus 836 AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
ATP + L-arginine + tRNAArgIII
-
 Saccharomyces pastorianus AMP + diphosphate + L-arginyl-tRNAArgIII
-
 ?
ATP + L-arginine + tRNAArgIII
-
 Saccharomyces pastorianus 836 AMP + diphosphate + L-arginyl-tRNAArgIII
-
 ?
additional information arginine-dependent ATP-diphosphate exchange Saccharomyces pastorianus ?
-
 ?
additional information arginine-dependent ATP-diphosphate exchange Saccharomyces pastorianus 836 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE Saccharomyces pastorianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 24 h, stable, without glycerol Saccharomyces pastorianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 aminoacylation Saccharomyces pastorianus