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6.1.1.19: arginine-tRNA ligase

This is an abbreviated version!
For detailed information about arginine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.19

Reaction

ATP
+
L-arginine
 +
tRNAArg
=
AMP
 +
diphosphate
 +
L-arginyl-tRNAArg

Synonyms

Arg-tRNA synthetase, Arginine translase, Arginine--tRNA ligase, Arginine-tRNA synthetase, Arginyl transfer ribonucleic acid synthetase, Arginyl-transfer RNA synthetase, Arginyl-tRNA synthetase, arginyl–tRNA synthetase, ArgRS, ArgS2, MtArgRS, RARS, RARS2, RRS, Synthetase, arginyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.19 arginine-tRNA ligase

Purification

Purification on EC 6.1.1.19 - arginine-tRNA ligase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
72000 MW form
-
affinity column chromatography, ion-exchange column chromatography, and Superdex 200 gel filtration
DEAE-Sepharose CL-6B column chromatography
-
DEAE-Sepharose CL-6B column chromatography, hydroxylapatite chromatography, Sepharose CL-2B column chromatography, and Sephacryl S-300 gel filtration
-
HiTrap Q column chromatography and Superdex 200 gel filtration
Ni-NTA column chromatography
Ni-NTA column chromatography and Source 15Q column chromatography
Ni-NTA column chromatography and Source15Q column chromatography
Ni-NTA Superflow resin chromatography and dialysis against 20 mM potassium phosphate buffer (pH 7.5)
-
Ni2+ column chromatography, Mono-Q column chromatography, and Superdex 200 gel filtration
Ni2+-NTA affinity resin column chromatography and Superdex 200 gel filtration
Ni2+-NTA agarose column chromatography
-
on a Ni2+-nitrilotriacetic superflow, a Resource Q, a Hitrap heparin, and a hydroxyapatite column
partial purification of recombinant enzymes using DEAE-Sepharose and Blue-Sepharose column chromatography
-
purification of a His-tagged recombinant enzyme using Ni-nitrilotriacetic acid agarose affinity chromatography
-
purification of the native and mutant enzymes expressed in Saccharomices cerevisiae, using Q-Sepharose and S-Sepharose chromatography
-
purification of the wild type and K116G mutant, expressed in Escherichia coli, by DEAE-Sephacel and PhenylSuperose column chromatography
-
recombinant GST-tagged MtArgRS from Escherichia coli strain BL21(DE3) by glutathione affinity chromatograophy. GST-MtArgRS shows the ability to co-purify with His-tagged MtSerRS by nickel affinity chromatography, co-elution of the two enzymes during gel filtration
-
recombinant His-tagged wild-type full-length and truncated mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration
-
soluble recombinant N-terminally HIS-/thioredoxin-tagged enzyme from Escherichia coli strain BL21 by metal affinity chromatography, tag cleavage by thrombin
two active forms which are interconvertible, I and II
-
using chromatography on Ni-nitrilotriacetic acid agarose
-
utilizing the His-tag, the tag is removed by thrombin treatment
utilizing the His-tag, the tag is removed by thrombin treatment, furthermore a Source15Q column is used