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6.1.1.19: arginine-tRNA ligase

This is an abbreviated version!
For detailed information about arginine-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.19

Reaction

ATP
+
L-arginine
 +
tRNAArg
=
AMP
 +
diphosphate
 +
L-arginyl-tRNAArg

Synonyms

Arg-tRNA synthetase, Arginine translase, Arginine--tRNA ligase, Arginine-tRNA synthetase, Arginyl transfer ribonucleic acid synthetase, Arginyl-transfer RNA synthetase, Arginyl-tRNA synthetase, arginyl–tRNA synthetase, ArgRS, ArgS2, MtArgRS, RARS, RARS2, RRS, Synthetase, arginyl-transfer ribonucleate

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.19 arginine-tRNA ligase

Engineering

Engineering on EC 6.1.1.19 - arginine-tRNA ligase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C599Y
-
expression in Saccharomices cerevisiae, shows reduced activity and stability
DELTA1-73
-
KM-value for tRNAArg is 1.2fold higher than the wild-type value, turnover number is 1.4fold higher
argS MA5002
-
mutant argS MA5002 differs from the wild-type ArgRS structural gene by one mutation, a substitution of an Arg by a Ser at position 134. It exhibits a 4times to 6times as low activity and a 5times as high Km value for ATP as the wild-type enzyme in aminoacylation and ATP-diphosphate exchange, Km values for Arg and tRNAArg remain unaltered
C320A
-
slight effect on activity
C537A
-
slight effect on activity
D118A
the mutation results in a 2fold increase in binding affinity
D317A
inactive
M460V
-
mutant cannot suppress the effect of the expression of FTOR126, a variant of the wild type tRNAArg
R324A
inactive
W162A
-
expression in Escherichia coli, 23% of wild-type activity
W172A
-
expression in Escherichia coli
W228A
-
expression in Escherichia coli
W349A
-
expression in Escherichia coli
W446A
-
expression in Escherichia coli
Y313A
the mutation results in a merely 2fold reduction in binding affinity
Y524D
-
mutant cannot suppress the effect of the expression of FTOR126, a variant of the wild type tRNAArg
G136K
-
inactive enzyme
K382A
-
half as active as the native enzyme
K385A
-
inactive enzyme
DELTANhcArgRS
-
N-terminal 72-amino acid deletion mutant with higher specific activity than the wild type enzyme
RARSL
-
the RARSL mutation in the mitochondrial arginyl–tRNA synthetase gene is associated with pontocerebellar hypoplasia
DELTANArgRS
lacking the N-terminal side from the 91st residue
K116G
-
expression of the mutant in Escherichia coli
N79D
can use tRNAArg with G20 as substrate
N79E
can use tRNAArg with G20 or U20 as substrate
N79K
can use tRNAArg with G20 as substrate
N79Q
can use tRNAArg with G20 as substrate
N79R
can use tRNAArg with G20 or U20 as substrate
Y77A
inactive mutant
Y77F
slight effect on activity
additional information