Cloned (Comment) | Organism |
---|---|
expression of the native and mutant enzymes in Saccharomyces cerevisiae | Cricetulus griseus |
Protein Variants | Comment | Organism |
---|---|---|
C599Y | expression in Saccharomices cerevisiae, shows reduced activity and stability | Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for ATP and L-arginine for several mutants and revertants | Cricetulus griseus | |
0.0007 | - |
L-arginine | ATP-diphosphate exchange reaction, pH 8, 25°C | Cricetulus griseus | |
0.0013 | - |
L-arginine | aminoacylation reaction, pH 8, 25°C | Cricetulus griseus | |
0.0023 | - |
L-arginine | ATP-diphosphate exchange reaction, pH 8, 37°C | Cricetulus griseus | |
0.0024 | - |
L-arginine | aminoacylation reaction, pH 8, 37°C | Cricetulus griseus | |
0.0032 | - |
tRNAArg | aminoacylation reaction, pH 8, 25°C | Cricetulus griseus | |
0.0044 | - |
tRNAArg | aminoacylation reaction, pH 8, 37°C | Cricetulus griseus | |
0.19 | - |
ATP | aminoacylation reaction, pH 8, 25°C | Cricetulus griseus | |
0.24 | - |
ATP | aminoacylation reaction, pH 8, 37°C | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of the native and mutant enzymes expressed in Saccharomices cerevisiae, using Q-Sepharose and S-Sepharose chromatography | Cricetulus griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity for different mutants | Cricetulus griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Cricetulus griseus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable for 6 h | Cricetulus griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values for ATP and L-arginine for several mutants and revertants | Cricetulus griseus |