Literature summary for 6.1.1.19 extracted from

Sekine, S.; Shimada, A.; Nureki, O.; Cavarelli, J.; Moras, D.; Vassylyev, D.G.; Yokoyama, S.
Crucial role of the high-loop lysine for the catalytic activity of arginyl-tRNA synthetase (2001), J. Biol. Chem., 276, 3723-3726.

Search for more literature for 6.1.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the wild type and K116G mutant in Escherichia coli	Thermus thermophilus

Protein Variants

Protein Variants	Comment	Organism
K116G	expression of the mutant in Escherichia coli	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of the wild type and K116G mutant, expressed in Escherichia coli, by DEAE-Sephacel and PhenylSuperose column chromatography	Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	K116G mutant displays very low activity compared to wild type enzyme	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-arginine + tRNAArg	-	Thermus thermophilus	AMP + diphosphate + L-arginyl-tRNAArg	-	?

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Release 2023.1 (January 2023)