Cloned (Comment) | Organism |
---|---|
construction of pMFT7H6-hcArgRS by inserting the full-length hcArgRS gene into the gap between NcoI and BamH1 of vector pMFT7H6. Expression of His-tagged wild-type full-length and truncated mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3)-RIL | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hemin | hemin is a protoporphyrin containing a ferric iron in the center. ArgRS Cys115 acts as a specific axial ligand of hemin binding that is located in the Add1 domain, but hemin binding to Cys115 is not responsible for the inhibition of enzymatic activity. Hemin inhibits the catalytic activity of full-length and N-terminal 72-amino acid-truncated hcArgRSs by blocking amino acid activation. Hemin induces oligomerization of both the isolated Add1 domain and the wild type enzyme, which might account for the inhibition of catalytic activity. Km values for tRNAArg, arginine, and ATP in the presence of hemin are not altered, but kcat values dramatically decrease compared with those in the absence of hemin, kinetic analysis, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values for tRNAArg, arginine, and ATP in the presence of hemin are not altered, but kcat values dramatically decrease compared with those in the absence of hemin, kinetic analysis, overview | Homo sapiens | |
0.0065 | - |
L-arginine | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin | Homo sapiens | |
0.0078 | - |
L-arginine | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin | Homo sapiens | |
0.0081 | - |
tRNAArg | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg | Homo sapiens | |
0.0096 | - |
tRNAArg | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg | Homo sapiens | |
0.501 | - |
ATP | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin | Homo sapiens | |
0.529 | - |
ATP | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | Homo sapiens | - |
AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type full-length and truncated mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nickel affinity chromatography and gel filtration | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of aminoacylation activity of Escherichia coli ArgRS and human truncated DELTANhcArgRS in presence of hemin, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Homo sapiens | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
ATP + L-arginine + tRNAArg | substrate is Escherichia coli tRNAArg | Homo sapiens | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Arginyl-tRNA synthetase | - |
Homo sapiens |
ArgRS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
tRNAArg | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg | Homo sapiens | |
3.4 | - |
ATP | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin | Homo sapiens | |
3.8 | - |
L-arginine | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin | Homo sapiens | |
15.5 | - |
ATP | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin | Homo sapiens | |
16.5 | - |
tRNAArg | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg | Homo sapiens | |
17.9 | - |
L-arginine | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the free form of human cytoplasmic arginyl-tRNA synthetase participates in ubiquitin-dependent protein degradation by offering arginyl-tRNAArg to arginyl-tRNA transferase, ATE1, overview | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
ATP | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin | Homo sapiens | |
30 | - |
ATP | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin | Homo sapiens | |
300 | - |
tRNAArg | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg | Homo sapiens | |
500 | - |
L-arginine | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin | Homo sapiens | |
1700 | - |
tRNAArg | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg | Homo sapiens | |
2800 | - |
L-arginine | pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin | Homo sapiens |