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Literature summary for 6.1.1.19 extracted from
- Arginyl-transfer ribonucleic acid synthetase of Bacillus stearothermophilus. Purification and kinetic analysis (1980), Eur. J. Biochem., 103, 169-177.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Geobacillus stearothermophilus |  |
| Periodate-oxidized tRNA | - |
Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00005 | 0.0006 | tRNAArg | pH 7.4 | Geobacillus stearothermophilus | |
| 0.001 | 0.002 | Arg | - |
Geobacillus stearothermophilus | |
| 1 | - |
ATP | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
gel filtration, sucrose density gradient centrifugation | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus NCA 1518 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-arginine + tRNAArg = AMP + diphosphate + L-arginyl-tRNAArg | mechanism includes the formation of an enzyme-bound aminoacyl-adenylate as an intermediate in the first step followed by transfer of the amino acid to tRNA | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine + tRNAArg | - |
Geobacillus stearothermophilus | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
| ATP + L-arginine + tRNAArg | - |
Geobacillus stearothermophilus NCA 1518 | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 59000, SDS-PAGE | Geobacillus stearothermophilus |
| More | cognate tRNA induces dimerization | Geobacillus stearothermophilus |
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