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Literature summary for 6.1.1.19 extracted from
- Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants (2009), FEBS J., 276, 1307-1318.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET32a for expression in Escherichia coli BL21 cells | Canavalia ensiformis |
| into the vector pET32a for expression in Escherichia coli BL21 cells | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
L-arginine | assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid | Glycine max |  |
| 0.003 | - |
L-arginine | assay method, aminoacylation of [32P]-labelled transcript tRNA | Glycine max | |
| 0.0078 | - |
L-arginine | assay method, aminoacylation of [32P]-labelled transcript tRNA | Canavalia ensiformis | |
| 0.0196 | - |
L-arginine | assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid | Canavalia ensiformis | |
| 0.0453 | - |
L-canavanine | assay method, aminoacylation of transcript tRNA with [14C]-labelled amino acid | Glycine max | |
| 0.0454 | - |
L-canavanine | assay method, aminoacylation of [32P]-labelled transcript tRNA | Glycine max | |
| 1.32 | - |
L-canavanine | assay method, aminoacylation of [32P]-labelled transcript tRNA | Canavalia ensiformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67400 | - |
theoretical | Canavalia ensiformis |
| 68200 | - |
theoretical | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine + tRNAArg | Canavalia ensiformis | - |
AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
| ATP + L-arginine + tRNAArg | Glycine max | - |
AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canavalia ensiformis | B2G3G6 | - |
- |
| Glycine max | B6ETP1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| utilizing the His-tag, the tag is removed by thrombin treatment | Canavalia ensiformis |
| utilizing the His-tag, the tag is removed by thrombin treatment, furthermore a Source15Q column is used | Glycine max |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
L-arginine, aminoacylation 100% | Canavalia ensiformis |
| additional information | - |
L-arginine, aminoacylation 100% | Glycine max |
| additional information | - |
L-canavanine, aminoacylation 15% | Canavalia ensiformis |
| additional information | - |
L-canavanine, aminoacylation 45% | Glycine max |
| additional information | - |
L-thioarginine, aminoacylation 82% | Glycine max |
| additional information | - |
L-thioarginine, aminoacylation 90% | Canavalia ensiformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine + tRNAArg | - |
Canavalia ensiformis | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
| ATP + L-arginine + tRNAArg | - |
Glycine max | AMP + diphosphate + L-arginyl-tRNAArg | - |
? | |
| ATP + L-canavanine + tRNAArg | - |
Canavalia ensiformis | AMP + diphosphate + L-canavanyl-tRNAArg | - |
? | |
| ATP + L-canavanine + tRNAArg | - |
Glycine max | AMP + diphosphate + L-canavanyl-tRNAArg | - |
? | |
| ATP + L-thioarginine + tRNAArg | - |
Canavalia ensiformis | AMP + diphosphate + L-thioarginyl-tRNAArg | - |
? | |
| ATP + L-thioarginine + tRNAArg | - |
Glycine max | AMP + diphosphate + L-thioarginyl-tRNAArg | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Arginyl-tRNA synthetase | - |
Canavalia ensiformis |
| Arginyl-tRNA synthetase | - |
Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
aminoacylation assay | Canavalia ensiformis |
| 30 | - |
aminoacylation assay | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
aminoacylation assay | Canavalia ensiformis |
| 7.5 | - |
aminoacylation assay | Glycine max |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Canavalia ensiformis | |
| ATP | - |
Glycine max | |
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