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4.2.2.10: pectin lyase

This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.

Word Map on EC 4.2.2.10

Reaction

[6-O-methyl-alpha-D-GalpA]n
=
[6-O-methyl-alpha-D-GalpA]m
+
4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1

Synonyms

commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.10 pectin lyase

Specific Activity

Specific Activity on EC 4.2.2.10 - pectin lyase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.316
-
crude enzyme, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C
0.38
-
40°C, pH 8.0
1.5
-
crude extract
12.9
-
after 8.6fold purification
1433
-
purified enzyme
148
-
recombinant enzyme in Escherichia coli cell supernatant
1502
-
purified recombinant enzyme
175
-
pH 8.0,temperature not specified in the publication
18.33
-
after 58.01fold purification, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C
26.85
-
pH 5.0, 43°C
297
AY825251
pH 10, 60°C
3.2
-
pH 9.0, 37°C
32
-
purified enzyme, McIlvaine buffer with 0.1 M citrate and 0.2 M phosphate
377
-
40°C, pH not specified in the publication
4.5
-
pH 7.0, 37°C
8.9
-
purified enzyme, acetate buffer 50 mM
additional information