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Literature summary for 4.2.2.10 extracted from

  • Yadav, S.; Yadav, P.K.; Yadav, D.; Yadav, K.D.
    Purification and characterization of an alkaline pectin lyase from Aspergillus flavus (2008), Process Biochem., 43, 547-552.
No PubMed abstract available
Search for more literature for 4.2.2.10

Activating Compound

Activating Compound Comment Organism Structure
Co2+ 25% increase of activity at 1 mM Aspergillus flavus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Aspergillus flavus
Ca2+ 88.3% residual activity at 1 mM Aspergillus flavus
Cu2+ complete inhibition at 1 mM Aspergillus flavus
EDTA 54.2% residual activity at 1 mM Aspergillus flavus
Hg2+ complete inhibition at 1 mM Aspergillus flavus
K+ 83.3% residual activity at 1 mM Aspergillus flavus
Mg2+ 87.5% residual activity at 1 mM Aspergillus flavus
additional information not influenced by Mn2+ Aspergillus flavus
Na+ 91.6% residual activity at 1 mM Aspergillus flavus
potassium ferrocyanide complete inhibition at 1 mM Aspergillus flavus
potassium permanganate complete inhibition at 1 mM Aspergillus flavus
Sodium arsenate 91.7% residual activity at 1 mM Aspergillus flavus
Sodium azide 75.0% residual activity at 1 mM Aspergillus flavus
Zn2+ 33.3% residual activity at 1 mM Aspergillus flavus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 SDS-PAGE Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
-
-
Aspergillus flavus MTCC 7589
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, DEAE cellulose chromatography and Sephadex G-100 gel filtration Aspergillus flavus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.316
-
 crude enzyme, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C Aspergillus flavus
18.33
-
 after 58.01fold purification, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the purified enzyme shows efficacy in retting of Crotalaria juncea fibers Aspergillus flavus ?
-
 ?
additional information the purified enzyme shows efficacy in retting of Crotalaria juncea fibers Aspergillus flavus MTCC 7589 ?
-
 ?
pectin
-
 Aspergillus flavus 4,5-unsaturated oligogalacturonates
-
 ?
pectin
-
 Aspergillus flavus MTCC 7589 4,5-unsaturated oligogalacturonates
-
 ?

Synonyms

Synonyms Comment Organism
PNL
-
 Aspergillus flavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme does not loose activity up to 50°C if exposed for 1 h, addition of ammonium sulfate in the range of 0.1-2.0 M increases the thermostability of the enzyme with 0.6 and 1.8 M of ammonium sulfate providing complete stability at 60 and 70°C. respectively, 40% of activity is recovered after incubating the purified enzyme at 60°C for 1 h, while there is 12% of activity recovered after incubation at 70°C for 1 h Aspergillus flavus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
52.2
-
 Pectin
-
 Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Aspergillus flavus

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Aspergillus flavus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 the enzyme is stable for 24 h in the pH range 4.0-10.0 Aspergillus flavus