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Literature summary for 4.2.2.10 extracted from
- Purification and characterization of pectin lyase secreted by Penicillium citrinum (2009), Biochemistry (Moscow), 74, 800-806.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Sodium arsenate | 122% relative activity at 1 mM | Penicillium citrinum |  |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the purified pectin lyase is efficient in retting of of natural fibers of mature stems of Linum usitatissimum, Cannabis sativa, and Crotalaria juncea | Penicillium citrinum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | no residual activity at 1 mM | Penicillium citrinum | |
| Cu2+ | 10% residual activity at 1 mM | Penicillium citrinum | |
| EDTA | 97% residual activity at 1 mM | Penicillium citrinum | |
| Hg2+ | 24% residual activity at 1 mM | Penicillium citrinum | |
| K+ | 91% residual activity at 1 mM | Penicillium citrinum | |
| K4[Fe(CN)6] | no residual activity at 1 mM | Penicillium citrinum | |
| KMnO4 | no residual activity at 1 mM | Penicillium citrinum | |
| Mg2+ | 97% residual activity at 1 mM | Penicillium citrinum | |
| Mn2+ | 76% residual activity at 1 mM | Penicillium citrinum | |
| Na+ | 97% residual activity at 1 mM | Penicillium citrinum | |
| Sodium azide | 91% residual activity at 1 mM | Penicillium citrinum | |
| Zn2+ | 76% residual activity at 1 mM | Penicillium citrinum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 114% relative activity at 1 mM | Penicillium citrinum | |
| additional information | Ca2+ and Co2+ have no significant effects on activity | Penicillium citrinum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
SDS-PAGE | Penicillium citrinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium citrinum | - |
- |
- |
| Penicillium citrinum MTCC 8897 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G 100 gel filtration and DEAE-cellulose column chromatography | Penicillium citrinum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
crude extract | Penicillium citrinum |
| 12.9 | - |
after 8.6fold purification | Penicillium citrinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin | - |
Penicillium citrinum | ? | - |
? | |
| pectin | - |
Penicillium citrinum MTCC 8897 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNL | - |
Penicillium citrinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Penicillium citrinum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
the pectin lyase is completely stable up to 40°C when exposed for 1 h, above 40°C it starts loosing activity | Penicillium citrinum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 76 | - |
Pectin | - |
Penicillium citrinum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
purified pectin lyase | Penicillium citrinum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 12 | the enzyme retains activity in the pH 9.0-12.0 range when exposed for 24 h | Penicillium citrinum |
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