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4.2.2.10: pectin lyase

This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.

Word Map on EC 4.2.2.10

Reaction

[6-O-methyl-alpha-D-GalpA]n
=
[6-O-methyl-alpha-D-GalpA]m
 +
4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1

Synonyms

commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.10 pectin lyase

Engineering

Engineering on EC 4.2.2.10 - pectin lyase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D154A
site-directed mutagenesis, reduced activity compared to the wild-type enzyme
D154E
site-directed mutagenesis, reduced activity compared to the wild-type enzyme
D186N
site-directed mutagenesis, reduced activity and altered pH profile compared to the wild-type enzyme
D186N/D221N
site-directed mutagenesis, reduced activity and altered pH profile compared to the wild-type enzyme
D221N
site-directed mutagenesis, reduced activity and altered pH profile compared to the wild-type enzyme
K239N
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
N109A
site-directed mutagenesis, reduced activity compared to the wild-type enzyme
R176A
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
R176D
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
R176K
site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme
R236A
site-directed mutagenesis, active site residue exchange, completely inactive mutant
R236K
site-directed mutagenesis, active site residue exchange, completely inactive mutant