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4.2.2.10: pectin lyase

This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.

Word Map on EC 4.2.2.10

Reaction

[6-O-methyl-alpha-D-GalpA]n
=
[6-O-methyl-alpha-D-GalpA]m
 +
4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1

Synonyms

commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.10 pectin lyase

Metals Ions

Metals Ions on EC 4.2.2.10 - pectin lyase

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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-mercaptoethanol
-
activates 1.5fold at 100 mM
Ag+
-
1 mM, 416% of initial activity
Ba2+
Erwinia aroidea
-
0.45 mM, slight stimulation
CaCl2
-
activates the purified enzyme by 50% at 1-100 mM, activates at 0.005% in the culture medium, activation is lowered in presence of MgSO4
Fe3+
-
up to 2.9fold activation
MgSO4
-
no effect on purified enzyme, activates at 0.005% in the culture medium, activation is lowered in presence of CaCl2
Sodium arsenate
-
slightly promotes the activity of PNL (110% relative activity at 1 mM)
Sr2+
Erwinia aroidea
-
0.45 mM, slight stimulation
Zn2+
-
1 mM, 130% of initial activity
additional information