Application | Comment | Organism |
---|---|---|
additional information | tremendous applications in textile industry, plant tissue maceration, and fruit juice waste water treatment | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates 1.5fold at 100 mM | Bacillus sp. (in: Bacteria) | |
CaCl2 | activates the purified enzyme by 50% at 1-100 mM, activates at 0.005% in the culture medium, activation is lowered in presence of MgSO4 | Bacillus sp. (in: Bacteria) | |
MgSO4 | no effect on purified enzyme, activates at 0.005% in the culture medium, activation is lowered in presence of CaCl2 | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
x * 106000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) DT7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
131.8fold | Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the bacterium is a soil isolate, optimization of culture medium conditions, overview | Bacillus sp. (in: Bacteria) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity at different culture conditions, U/ml | Bacillus sp. (in: Bacteria) |
1433 | - |
purified enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
pectin | - |
Bacillus sp. (in: Bacteria) DT7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 106000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
pectin lyase | - |
Bacillus sp. (in: Bacteria) |
pectinase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, partially stable | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | enzyme is optimally stable at pH 7.5-8.5, 50% activity at neutral pH, complete loss of activity occurs below pH 6.0 and above pH 9.0 | Bacillus sp. (in: Bacteria) |