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4.2.2.10: pectin lyase

This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.

Word Map on EC 4.2.2.10

Reaction

[6-O-methyl-alpha-D-GalpA]n
=
[6-O-methyl-alpha-D-GalpA]m
 +
4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1

Synonyms

commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.10 pectin lyase

Inhibitors

Inhibitors on EC 4.2.2.10 - pectin lyase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
-
Ag2+
-
complete inhibition
Al3+
-
10 mM, 5% residual activity
arsenate
-
1 mM, 92% of initial activity
CaCl2
-
slight inhibition at 100 mM
Cinnamic acid
-
enzyme activitiy of pectin lyase decreases significantly when 0.1 mg/l of cinnamlic acid is added
Citric acid
-
1 mM, 78% of initial activity
coumaric acid
-
enzyme activitiy of pectin lyase decreases significantly when 0.1 mg/l of coumaric acid is added
diethyldicarbonate
-
1 mM, complete inactivation
dithiothreitol
-
-
endogenous enzyme inhibitor protein
-
MW of 25 kDa, loss of 70% activity after treatment with trypsin, unaffected by DNase I and RNase A, loss of 65% activity after heating to 95°C for 5 min
-
ferrocyanide
-
1 mM, complete loss of activity
ferulic acid
-
enzyme activitiy of pectin lyase decreases significantly when 0.1 mg/l of ferulic acid is added
formate
-
-
gallic acid
-
enzyme activitiy of pectin lyase decreases significantly when 0.1 mg/l of gallic acid is added
H2O2
-
5 mM, 29% inhibition
Iodine
iodoacetate
-
-
iso-butyrate
-
-
K4[Fe(CN)6]
KMnO4
Li+
-
10 mM, 40% inhibition
malic anhydride
-
2 mM, complete inhibition
N-bromosuccinimide
n-Butyrate
-
-
N-Chlorosuccinimide
-
2 mM, 21% inhibition
NAD+
-
above 0.25 mM
NEM
-
2 mM, 96% inhibition
p-chloromercuriphenylsulfonic acid
-
2 mM, complete inhibition
Pectin
-
substrate inhibition
Permanganate
-
1 mM, complete loss of activity
Phenylmethylsulfonylfluoride
potassium ferrocyanide
potassium permanganate
propionate
-
-
SDS
-
10 mM, more than 50% inhibition
Sodium arsenate
Sodium azide
Tannic acid
-
-
trans-caffeate
-
enzyme activitiy of pectin lyase decreases significantly when 0.1 mg/l of caffic acid is added
additional information
-