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Literature summary for 4.2.2.10 extracted from
- Overexpression of the pectin lyase gene of Pseudomonas marginalis in Escherichia coli and purification of the active enzyme (2003), Biotechnol. Appl. Biochem., 37, 187-194.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pnl, functional overexpression of the soluble His-tagged enzyme in Escherichia coli BL21(DE3) | Pseudomonas marginalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Pseudomonas marginalis |  |
| endogenous enzyme inhibitor protein | MW of 25 kDa, loss of 70% activity after treatment with trypsin, unaffected by DNase I and RNase A, loss of 65% activity after heating to 95°C for 5 min | Pseudomonas marginalis | |
| additional information | enzyme is unaffeced by 5% v/v glycerol | Pseudomonas marginalis | |
| NAD+ | above 0.25 mM | Pseudomonas marginalis | |
| NADH | - |
Pseudomonas marginalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Pseudomonas marginalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas marginalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification of native enzyme, recombinant His-tagged enzyme from Escherichia coli, 113fold to near homogeneity via solubilization and renaturation of inclusion bodies, large DNA fragments prove to be contaminating by binding to the enzyme | Pseudomonas marginalis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| renaturation with 8 M urea of recombinant His-tagged enzyme expressed in inclusion bodies in Escherichia coli | Pseudomonas marginalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 148 | - |
recombinant enzyme in Escherichia coli cell supernatant | Pseudomonas marginalis |
| 1502 | - |
purified recombinant enzyme | Pseudomonas marginalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme binds DNA | Pseudomonas marginalis | ? | - |
? | |
| pectin | - |
Pseudomonas marginalis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pectin lyase | - |
Pseudomonas marginalis |
| PNL | - |
Pseudomonas marginalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
native enzyme | Pseudomonas marginalis |
| 37 | - |
recombinant His-tagged enzyme | Pseudomonas marginalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
Pectin | pH 6.5, 37°C | Pseudomonas marginalis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Pseudomonas marginalis |
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