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Literature summary for 4.2.2.10 extracted from
- Pectin lyase from Aspergillus giganteus: Comparative study of productivity of submerged fermentation on citrus pectin and orange waste (2009), Appl. Biochem. Microbiol., 45, 610-616.
No PubMed abstract available
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus giganteus | - |
- |
- |
| Aspergillus giganteus CCT 3232 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activity of pectin lyase using citrus pectin as substrate is 60.891 units/mg, the specific activity of pectin lyase using orange waste as substrate is 38.364 units/mg, the specific activity of pectin lyase using polygalacturonic acid as substrate is 35.86 units/mg, the specific activity of pectin lyase using galacturonic acid as substrate is 63.403 units/mg, one unit of activity is defined as the amount of enzyme causing a rise in 0.010 absorbance units per h | Aspergillus giganteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin | - |
Aspergillus giganteus | ? | - |
? |  |
| citrus pectin | - |
Aspergillus giganteus CCT 3232 | ? | - |
? | |
| galacturonic acid | - |
Aspergillus giganteus | ? | - |
? | |
| galacturonic acid | - |
Aspergillus giganteus CCT 3232 | ? | - |
? | |
| orange waste | under optimum conditions, the highest pectin lyase activity in the medium is with orange waste as carbon source | Aspergillus giganteus | ? | - |
? | |
| orange waste | under optimum conditions, the highest pectin lyase activity in the medium is with orange waste as carbon source | Aspergillus giganteus CCT 3232 | ? | - |
? | |
| polygalacturonic acid | - |
Aspergillus giganteus | ? | - |
? | |
| polygalacturonic acid | - |
Aspergillus giganteus CCT 3232 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus giganteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | the pectin lyase activity shows thermal stability, with half-lives of 30 and 27 min when incubated at 45 and 50°C, respectively | Aspergillus giganteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Aspergillus giganteus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | high stability is observed at room temperature from pH 6.0 to 10.0 with more than 85% of enzyme activity preserved | Aspergillus giganteus |
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Release 2023.1 (January 2023)
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