4.2.1.84: nitrile hydratase

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For detailed information about nitrile hydratase, go to the full flat file.

Reaction

Synonyms

3-cyanopyridine hydratase, acrylonitrile hydratase, aliphatic nitrile hydratase, ANHase, Co-type NHase, Co-type nitrile hydratase, cobalt-containing nitrile hydratase, CoIII-NHase, CtNHase, Fe-NHase, H-NHase, H-nitrilase, high-molecular mass nitrile hydratase, high-molecular weight nitrile hydratase, hydratase, nitrile, iron-type nitrile hydratase, L-Nhase, L-nitrilase, low-molecular mass nitrile hydratase, low-molecular weight nitrile hydratase, MbNHase, NHase, NHaseK, NI1 NHase, NilCo, NilFe, nitrilase, nitrile hydratase, NthAB, PaNit, ppNHase, ReNHase, TNHase, toyocamycin nitrile hydratase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.84 nitrile hydratase

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Results	in table
24	Activating Compound
6842	AA Sequence
82	Application
1	CAS Registry Number
57	Cloned(Commentary)
8	Cofactor
25	Crystallization (Commentary)
33	Disease/ Diagnostics
15	Expression
19	General Information
14	General Stability
19	IC50 Value
218	Inhibitors
51	kcat/KM [mM/s]
39	Ki Value [mM]
181	KM Value [mM]
5	Localization
103	Metals/Ions
108	Molecular Weight [Da]
111	Natural Substrates/ Products (Substrates)
16	Organic Solvent Stability
815	Organism
1	Oxidation Stability
14	Pathway
159	PDB ID
71	pH Optimum
22	pH Range
12	pH Stability
3	pI Value
4	Posttranslational Modification
106	Protein Variants
58	Purification (Commentary)
27	Reaction
8	Reaction Type
340	Reference
9	Source Tissue
131	Specific Activity [micromol/min/mg]
10	Storage Stability
831	Substrates and Products (Substrate)
110	Subunits
195	Synonyms
1	Systematic Name
70	Temperature Optimum [°C]
14	Temperature Range [°C]
69	Temperature Stability [°C]
75	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 4.2.1.84 - nitrile hydratase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 2 weeks, 90% residual activity, with addition of 8% glycerol, complete loss of activity within one week

Rhodococcus rhodochrous

-

666090

-20°C, 50% glycerol, 0.01 M HEPES/KOH buffer, pH 7.2, 22 mM n-butyric acid, stable for more than 2 months

Pseudomonas chlororaphis

-

648440

-20°C, pH 7.0, 50% glycerol, stable for at least 40 days, 40% loss of activity after 6 months

Arthrobacter sp.

-

648422

-40ºC, pH 7.2, 40 mM butyric acid, stable

Comamonas testosteroni

-

648443

-80°C, stable for weeks

Bacillus sp. (in: Bacteria)

-

666930

0°C, pH 7.0, 50% loss of activity after 40 days

Arthrobacter sp.

-

648422

4°C, purified recombinant wild-type enzyme, retains complete activity for 1 year, and the catalytic center does not change as confimed by analysis of the crystal structure

Pseudonocardia thermophila

Q7SID2, Q7SID3

648457

4°C, stable for weeks

Bacillus sp. (in: Bacteria)

-

666930

4°C, Tris-HCl buffer pH 7.2

Nocardia sp.

-

671402

organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid

Rhodococcus rhodochrous

-

648439