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4.2.1.84: nitrile hydratase

This is an abbreviated version!
For detailed information about nitrile hydratase, go to the full flat file.

Word Map on EC 4.2.1.84

Reaction

an aliphatic amide
=
A nitrile
+
H2O

Synonyms

3-cyanopyridine hydratase, acrylonitrile hydratase, aliphatic nitrile hydratase, ANHase, Co-type NHase, Co-type nitrile hydratase, cobalt-containing nitrile hydratase, CoIII-NHase, CtNHase, Fe-NHase, H-NHase, H-nitrilase, high-molecular mass nitrile hydratase, high-molecular weight nitrile hydratase, hydratase, nitrile, iron-type nitrile hydratase, L-Nhase, L-nitrilase, low-molecular mass nitrile hydratase, low-molecular weight nitrile hydratase, MbNHase, NHase, NHaseK, NI1 NHase, NilCo, NilFe, nitrilase, nitrile hydratase, NthAB, PaNit, ppNHase, ReNHase, TNHase, toyocamycin nitrile hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.84 nitrile hydratase

Molecular Weight

Molecular Weight on EC 4.2.1.84 - nitrile hydratase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
101000
-
sedimentation equilibrium, low molecular mass enzyme, L-NHase
102000
-
gel filtration
110000
130000
-
gel filtration, low molecular mass enzyme, L-NHase
146000
-
enzyme 2
22700
22800
22953
-
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation
22975
-
alphabeta, 1 * 22975 + 1 * 23493, MALDI-TOF MS
22982
23000
23486
-
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation
23493
-
alphabeta, 1 * 22975 + 1 * 23493, MALDI-TOF MS
24000
24108
-
2 * 22982, alpha + 2 * 24108, beta, amino acid sequence, gel filtration
242000
-
enzyme 1
25000
25040
-
1 * 25040 + 1 * 30600, estimated from SDS-PAGE
25400
-
2 * 25400, alpha-subunit, + 2 * 26700, beta-subunit, calculated
26000
26700
-
2 * 25400, alpha-subunit, + 2 * 26700, beta-subunit, calculated
26900
-
2 * 26900, SDS-PAGE
27000
27500
27700
-
2 * 27700
278000
28000
-
x * 25000 + x * 28000, SDS-PAGE
28500
-
x * 28500, alpha + x * 29000, beta, SDS-PAGE, N774 strain
29000
30600
-
1 * 25040 + 1 * 30600, estimated from SDS-PAGE
32000
-
x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7
364000
-
proposal for holoenzyme subunit composition: alpha2 beta4, based on calibrated gel filtration and absorption spectra
420000
505000 - 530000
52000
520000
-
high molecular mass enzyme, H-NHase
54000
-
chromatography on Sephacryl S-400 and Sepharose CL-6B, alpha-beta form
55746
-
alpha,beta2, 1 * 55746 + 2 * 63001, 32% amino acid sequence identity of subunits, MALDI-TOF MS and HPLC
61400
63001
-
alpha,beta2, 1 * 55746 + 2 * 63001, 32% amino acid sequence identity of subunits, MALDI-TOF MS and HPLC
70000
72000
-
x * 26000 + x * 72000, SDS-PAGE
77000
-
gel filtration on Sephadex-G150
80000
84000
-
gel filtration
85000
86000
-
holoenzyme, native PAGE
87000
-
gel filtration, HPLC
90000
-
gel filtration
90000 - 94000
92300
-
gel filtration
94000
95000
-
4 * 95000