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4.2.1.84: nitrile hydratase

This is an abbreviated version!
For detailed information about nitrile hydratase, go to the full flat file.

Word Map on EC 4.2.1.84

Reaction

an aliphatic amide
=
A nitrile
 +
H2O

Synonyms

3-cyanopyridine hydratase, acrylonitrile hydratase, aliphatic nitrile hydratase, ANHase, Co-type NHase, Co-type nitrile hydratase, cobalt-containing nitrile hydratase, CoIII-NHase, CtNHase, Fe-NHase, H-NHase, H-nitrilase, high-molecular mass nitrile hydratase, high-molecular weight nitrile hydratase, hydratase, nitrile, iron-type nitrile hydratase, L-Nhase, L-nitrilase, low-molecular mass nitrile hydratase, low-molecular weight nitrile hydratase, MbNHase, NHase, NHaseK, NI1 NHase, NilCo, NilFe, nitrilase, nitrile hydratase, NthAB, PaNit, ppNHase, ReNHase, TNHase, toyocamycin nitrile hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.84 nitrile hydratase

pH Range

pH Range on EC 4.2.1.84 - nitrile hydratase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 12
-
pH profile of soluble and immobilized enzymes, overview
5 - 8
-
about 50% of activity maximum at pH 5, about 60% of activity maximum at pH 8
5 - 9
-
about 60% of activity maximum at pH 5, about 55% of activity maximum at pH 9
5.1 - 8.7
-
about 50% of maximum activity
5.5 - 10
-
activity drops sharply below pH 5.5 and above pH 10.0
5.5 - 11
Corynebacterium nitrilophilus
-
sharp drop of activity below pH 5.5, moderate decrease of activity at pH 8-11
5.5 - 9
pH 5.5: about 60% of maximal activity, pH 9.0: about 60% of maximal activity
6 - 10
6 - 8
-
-
6 - 8.3
-
about 50% of activity maximum at pH 6, about 35% of activity maximum at pH 8.3
6 - 8.5
-
H-NHase
6 - 9
6.4 - 7.6
-
pH 4.0: no NHase activity
7 - 8.5
-
pH 7.0: about 80% of maximal activity, pH 8.5: about 75% of maximal activity
7.2 - 7.6
-
hydration of acrylonitrile at 25°C, rapid decrease of activity below pH 6.4
additional information
-
different buffer systems including phthalate, phosphate and borax buffer are tested for influence on hydration of acrylonitrile