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Literature summary for 4.2.1.84 extracted from

  • Wang, Y.J.; Zheng, Y.G.; Zheng, R.C.; Shen, Y.C.
    Stability study on the nitrile hydratase of Nocardia sp. 108: From resting cells to crude enzyme preparation (2006), Appl. Biochem. Microbiol., 42, 384-387.
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General Stability

General Stability Organism
KH2PO4-NaOH and Tris-HCl buffer are tested at 28°C and pH 7.5. 20% NHase activity is lost after 18 h and is further decreasing. 73.5 h later, NHase activity in Tris-HCl is unchanged while in KH2PO4-NaOH abrupt decrease is observed. Nocardia sp.

Organism

Organism UniProt Comment Textmining
Nocardia sp.
-
-
-
Nocardia sp. 108
-
-
-

Purification (Commentary)

Purification (Comment) Organism
(NH4)2SO4 fractionation Nocardia sp.

Storage Stability

Storage Stability Organism
4°C, Tris-HCl buffer pH 7.2 Nocardia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
 Nocardia sp. acrylamide
-
 ?
acrylonitrile + H2O
-
 Nocardia sp. 108 acrylamide
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 24
-
 Nocardia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Nocardia sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Nocardia sp.