Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.1.84: nitrile hydratase

This is an abbreviated version!
For detailed information about nitrile hydratase, go to the full flat file.

Word Map on EC 4.2.1.84

Reaction

an aliphatic amide
=
A nitrile
 +
H2O

Synonyms

3-cyanopyridine hydratase, acrylonitrile hydratase, aliphatic nitrile hydratase, ANHase, Co-type NHase, Co-type nitrile hydratase, cobalt-containing nitrile hydratase, CoIII-NHase, CtNHase, Fe-NHase, H-NHase, H-nitrilase, high-molecular mass nitrile hydratase, high-molecular weight nitrile hydratase, hydratase, nitrile, iron-type nitrile hydratase, L-Nhase, L-nitrilase, low-molecular mass nitrile hydratase, low-molecular weight nitrile hydratase, MbNHase, NHase, NHaseK, NI1 NHase, NilCo, NilFe, nitrilase, nitrile hydratase, NthAB, PaNit, ppNHase, ReNHase, TNHase, toyocamycin nitrile hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.84 nitrile hydratase

IC50 Value

IC50 Value on EC 4.2.1.84 - nitrile hydratase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
2-Nitrophenol
Comamonas testosteroni
-
+/- 0.001, NilFe
0.01
3-Nitrophenol
Comamonas testosteroni
-
+/- 0.002, NilFe
0.0086 - 0.01
4-nitrophenol
0.003 - 0.004
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
0.005 - 0.009
acrylonitrile
0.3 - 0.4
Butyric acid
2
hexanoic acid
Brevibacterium sp.
-
IC50 of 2 mM
0.003 - 0.005
KCN
12
n-butyric acid
Brevibacterium sp.
-
competitive inhibitor. Complete inhibition beyond 40 mM. IC50 of 4 mM at pH 7.2 in Hepes buffer 100 mM, IC50 of 8 mM at pH 7.2 in sodium phosphate buffer 100 mM, IC50 of 0.8 mM at pH 6.2 in sodium phosphate buffer 100 mM, IC50 of 12 mM at pH 8.2 in sodium
0.008 - 0.5
NaNO2
0.007 - 0.1
tertiary-butyl isonitrile
0.5
Valeric acid
Brevibacterium sp.
-
IC50 of 0.5 mM