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4.2.1.84: nitrile hydratase

This is an abbreviated version!
For detailed information about nitrile hydratase, go to the full flat file.

Word Map on EC 4.2.1.84

Reaction

an aliphatic amide
=
A nitrile
 +
H2O

Synonyms

3-cyanopyridine hydratase, acrylonitrile hydratase, aliphatic nitrile hydratase, ANHase, Co-type NHase, Co-type nitrile hydratase, cobalt-containing nitrile hydratase, CoIII-NHase, CtNHase, Fe-NHase, H-NHase, H-nitrilase, high-molecular mass nitrile hydratase, high-molecular weight nitrile hydratase, hydratase, nitrile, iron-type nitrile hydratase, L-Nhase, L-nitrilase, low-molecular mass nitrile hydratase, low-molecular weight nitrile hydratase, MbNHase, NHase, NHaseK, NI1 NHase, NilCo, NilFe, nitrilase, nitrile hydratase, NthAB, PaNit, ppNHase, ReNHase, TNHase, toyocamycin nitrile hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.84 nitrile hydratase

Activating Compound

Activating Compound on EC 4.2.1.84 - nitrile hydratase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acrylonitrile
-
below
activator 17K
the putative activator (17K, MW 17 kDa) gene next to the beta-subunit gene is proven to be important for the functional expression of recombinant NHaseK in Escherichia coli
-
crotonamide
-
induces high and low molecular mass forms of the enzyme
cyclohexanecarboxamide
-
induces the low molecular mass form of the enzyme
dimethylformamide
-
addition to increase the accessibility of nitrile groups at concentration no higher than 0.5% is beneficial for activity
glycine-NaOH
-
activates
GroEL-ES
-
Escherichia coli chaperones GroEL-ES work well in Rhodococcus and simultaneously possess protein-folding assistance functions and the ability to stabilize and reactivate the native nitrile hydratase
-
light
-
methacrylamide
-
induces the enzyme
NHase activator protein
the activator gene encding activator protein might be involved in protein folding of the alpha- and beta-subunits of NHase
-
nitric oxide
-
the inactive (dark form) nitric oxide-bound enzyme is activated when exposed to light via the release of nitric oxide from the iron center
nitrile hydratase activator protein
residue aTyr114 may be involved in the interaction with the nitrile hydratase activator protein of Pseudomonas thermophila
-
P14 protein
-
protein encoded in an open frame of the structural frames that is essential for optimal activity of NHase over-produced in Escherichia coli
-
potassium hexacyanoferrate
-
oxidizing agent that activates the Co-substituted enzyme by oxidizing the Co2+ atom and/or modification of a alphaCys-112 to cysteine-sulfinic acid
additional information
-