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Literature summary for 4.2.1.84 extracted from

  • Wang, C.; Zhang, G.; Xu, X.; Li, C.
    Inducing expression and reaction characteristic of nitrile hydratase from Rhodococcus sp. SHZ-1 (2007), Chin. J. Chem. Engin., 15, 573-578.
No PubMed abstract available
Search for more literature for 4.2.1.84

Activating Compound

Activating Compound Comment Organism Structure
acrylonitrile below Rhodococcus sp.
NH4Cl
-
 Rhodococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
Acrylamide
-
 Rhodococcus sp.
acrylonitrile above > 40 g/l Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
95
-
 acrylonitrile
-
 Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. SHZ-1
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.82
-
 acrylamide (500 g/l) Rhodococcus sp.
12.3
-
 acrylonitrile (100 g/l) Rhodococcus sp.
20.6
-
 acrylamide (100 g/l) Rhodococcus sp.
27.99
-
 acrylonitrile (30 g/l) Rhodococcus sp.
924
-
 control, no inducer Rhodococcus sp.
1024
-
 inducer: NH4Cl (75 mM) Rhodococcus sp.
1136
-
 inducer: acrylonitrile (50 mM) Rhodococcus sp.
1358
-
 inducer: NH4Cl (75 mM) and acrylonitrile (50 mM) Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
 Rhodococcus sp. acrylamide
-
 ?
acrylonitrile + H2O
-
 Rhodococcus sp. SHZ-1 acrylamide
-
 ?

Synonyms

Synonyms Comment Organism
NHase
-
 Rhodococcus sp.
nitrile hydratase
-
 Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 Arrhenius plot: activation energy: 90.2 kJ/mol (substrate acrylonitrile), evaluation in the temperatur range from 5 to 30 °C Rhodococcus sp.
30
-
-
 Rhodococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 half-lives: 40°C: 2.3 h, 50°C: 0.98 h, 25°C: 6.31 h, 35°C: 5.45 h Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Rhodococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.4 7.6 pH 4.0: no NHase activity Rhodococcus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
283
-
 acrylonitrile
-
 Rhodococcus sp.