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4.2.1.84: nitrile hydratase

This is an abbreviated version!
For detailed information about nitrile hydratase, go to the full flat file.

Word Map on EC 4.2.1.84

Reaction

an aliphatic amide
=
A nitrile
+
H2O

Synonyms

3-cyanopyridine hydratase, acrylonitrile hydratase, aliphatic nitrile hydratase, ANHase, Co-type NHase, Co-type nitrile hydratase, cobalt-containing nitrile hydratase, CoIII-NHase, CtNHase, Fe-NHase, H-NHase, H-nitrilase, high-molecular mass nitrile hydratase, high-molecular weight nitrile hydratase, hydratase, nitrile, iron-type nitrile hydratase, L-Nhase, L-nitrilase, low-molecular mass nitrile hydratase, low-molecular weight nitrile hydratase, MbNHase, NHase, NHaseK, NI1 NHase, NilCo, NilFe, nitrilase, nitrile hydratase, NthAB, PaNit, ppNHase, ReNHase, TNHase, toyocamycin nitrile hydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.84 nitrile hydratase

Turnover Number

Turnover Number on EC 4.2.1.84 - nitrile hydratase

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.74
(R)-mandelonitrile
pH 7.4, 10°C, wild-type enzyme
1.2 - 1.4
(S)-mandelonitrile
145.6
2-cyanobenzamide
pH 7.4, 35°C, wild-type enzyme
443.3
2-Cyanopyridine
-
soluble NHase, at 50°C
17.3 - 8935.6
3-Cyanopyridine
107.7
4-cyanobenzamide
pH 7.4, 35°C, wild-type enzyme
59.05 - 90
4-cyanopyridine
19.6 - 435.7
5-cyanovaleramide
1.04
acetamiprid
pH 7.5, 30°C
12.4 - 41
acetonitrile
2 - 1910
acrylonitrile
4.9 - 1489.82
Benzonitrile
0.99
Butyronitrile
-
+/- 0.04, 10 mM HEPES, pH 7.5, 37°C
39.1 - 417.2
cyanoacetamide
426.68
indole-3-acetonitrile
pH 7.5, 30°C
0.0000065 - 55000
Methacrylonitrile
0.0035 - 0.327
n-valeronitrile
2.6 - 30.7
Propionitrile
131.36
thiacloprid
pH 7.5, 30°C
0.44 - 159
Toyocamycin
76.3
Valeronitrile
-
-