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3.8.1.5: haloalkane dehalogenase

This is an abbreviated version!
For detailed information about haloalkane dehalogenase, go to the full flat file.

Word Map on EC 3.8.1.5

Reaction

1-Haloalkane
+
H2O
=
a primary alcohol
 +
halide

Synonyms

1,3,4,6,-tetrachloro-1,4-cyclohexadiene halidohydrolase, 1-chlorohexane halidohydrolase, 1-haloalkane dehalogenase, DadB, DatA, DbeA, DbjA, DccA, DhaA, DhaA31, DhaB, DhaC, DhAf, DhlA, DhmA, DmaA, dmbA, DmbB, DmbC, dmlA, DmmA, DmrA, DmrB, DmsA, DmtA, DmxA, DpcA, DppA, DrbA, DsaA, DspA, EC 3.8.1.1, eHLD-B, eHLD-C, haloalkane dehalogenase, haloalkane dehalogenase LinB, HanR, HLD, HLD-I, LinB, LinBMI, LinBUT, metallo-haloalkane dehalogenase, protein XP_504164, Rv2579, Ylehd

ECTree

     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.5 haloalkane dehalogenase

Cloned

Cloned on EC 3.8.1.5 - haloalkane dehalogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminally His-tagged enzyme is expressed in Escherichia coli BL21(DE3) ArcticExpress cells
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in leaves of Nicotiana tabacum line Xanthi
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21 Star (DE3) cells
-
expression in Escherichia coli BL21(DE3)
expression in Escherichia coli BL21(DE3) cells
expression of wild-type and mutant enzymes in Escherichia coli
-
gene dadB, phylogenetic analysis
gene datA, DNA and amnino acid sequence determination and analysis
gene datA, phylogenetic analysis
-
gene dbeA, phylogenetic analysis
-
gene dbjA, phylogenetic analysis
-
gene dhaA, expression of a saturation mutagenesis library in Escherichia coli strain BL21(DE3)
gene dhaA, phylogenetic analysis
-
gene dhlA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overexpression in Escherichia coli strain BL21(DE3)
gene dhlA, expression of a saturation mutagenesis library in Escherichia coli strain BL21(DE3)
gene dhlA, phylogenetic analysis
-
gene dmmA from cosmid pLM17, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutants in Escherichia coli strain BL21(DE3)
-
gene dmrA, sequence comparisons and phylogenetic analysis, overexpression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene dmrB, sequence comparisons and phylogenetic analysis, overexpression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene dmxA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene dpcA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene dppA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21-Gold(DE3)
gene drbA, phylogenetic analysis
-
gene dspA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene linB, DNA and amino acid sequence determination and analysis, genotyping and sequence comparisons
-
gene linB, DNA and amino acid sequence determination and analysis, genotyping and sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene linB, expression of His6-tagged LinB in Escherichia coli strain BL21 AI
-
gene linB, phylogenetic analysis
-
genes dmbA and dmbC, phylogenetic analysis
-
genotyping, DNA and amino acid sequence determination and analysis, analysis of the composition and diversity of metagenomic sequences encoding alpha/beta-hydrolase fold proteins in four distinct mangrove soils
GJ10 mutant H289Q, expression in Escherichia coli
-
HanR, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3) Rosetta2
-
into pGEX-5X-3 for expression in Escherichia coli BL21 cells
multiple alignment of protein sequences of cloned haloalkane dehalogenases
mutant enzyme H272F is expressed in Escherichia coli BL21 cells
-
mutant enzyme H280F is expressed in Escherichia coli BL21 cells
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli JM109
-
phylogenetic analysis and tree
recombinant expression as N-terminally His-tagged enzyme in Escherichia coli TOP10
recombinant expression of His-tagged wild-type and mutant enzymes
-
recombinant overexpression of wild-tye and mutant enzymes in Escherichia coli strain Rosetta (DE3)
the coding sequence is amplified in the pAL-781-dhmA vector, the vector pUC18-dhmAHis is propagated in Escherichia coli JM110, the dhmAHis gene is ligated into the vector pK4RP for expression in Rhodococcus erythropolis
the ORF of Rv2579 is inserted into the cloning vector pDONR-201, the resulting vector is used to transfer the coding sequence into the expression vector pDET-15
the plasmid pYBJA2 is used for expression of the protein in Escherichia coli BL21 cells, the DbjA construct codes for residues 1-310 of DbjA with its C-terminus fused to a His6-tag
-
the recombinant His-tagged enzyme is expressed in Escherichia coli BL21AI
using the plasmids pKS13, pUC18, pUC19 and pAQN, the plasmid pAQN is used for epression of the protein in Escherichia coli BL21 cells
using the plasmids pKS13, pUC18, pUC19 and pAQNM, the plasmid pAQNM is used for expression of the protein in Escherichia coli BL21 cells
UT26, His-tagged protein expressed in Escherichia coli
-
UT26, overexpression in Escherichia coli
-