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Literature summary for 3.8.1.5 extracted from

  • Prudnikova, T.; Mozga, T.; Rezacova, P.; Chaloupkova, R.; Sato, Y.; Nagata, Y.; Brynda, J.; Kuty, M.; Damborsky, J.; Smatanova, I.K.
    Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94 (2009), Acta Crystallogr. Sect. F, 65, 353-356.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Bradyrhizobium elkanii

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type DbeA and mutant enzyme DbeA1, which carries the unique fragment of DbjA, are crystallized using the sitting-drop vapour-diffusion method, in 100 mM Tris-HCl buffer pH 7.5, containing 25% (w/v) PEG 3350 and 130 mM calcium acetate, at 4°C and 20°C Bradyrhizobium elkanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 SDS-PAGE Bradyrhizobium elkanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bradyrhizobium elkanii DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA ?
-
 ?
additional information Bradyrhizobium elkanii USDA94 DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium elkanii
-
-
-
Bradyrhizobium elkanii USDA94
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose column chromatography Bradyrhizobium elkanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA Bradyrhizobium elkanii ?
-
 ?
additional information DbeA is less active and has a higher specificity towards brominated and iodinated compounds compared with DbjA Bradyrhizobium elkanii USDA94 ?
-
 ?

Synonyms

Synonyms Comment Organism
DbeA
-
 Bradyrhizobium elkanii