Literature summary for 3.8.1.5 extracted from

Tratsiak, K.; Degtjarik, O.; Drienovska, I.; Chrast, L.; Rezacova, P.; Kuty, M.; Chaloupkova, R.; Damborsky, J.; Kuta Smatanova, I.
Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17 (2013), Acta Crystallogr. Sect. F, 69, 683-688.

Cloned(Commentary)

Cloned (Comment)	Organism
gene dmxA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Marinobacter sp.
gene dpcA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Psychrobacter cryohalolentis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme free or in complex with ligand 1,2-dichloroethane, sitting or haning drop vapour diffusion technique, mixing 0.002 ml protein solution and 0.001 ml precipitant solution plus 300-600 nl 0.1 M L-proline or 0.1 M sarcosine, optimization of crystallization conditions, DmxA complex with 1,2-dichloroethane obtained by soaking experiments after the addition of 0.05 ml 1,2-dichloroethane to the reservoir well containing 0.8 ml of the precipitating solution with the crystals and incubation for 7 days, 19°C, X-ray diffraction structure determination and analysis at 2.10-2.49 A resolution	Marinobacter sp.
purified enzyme free or in complex with ligands 1-bromohexane and 1,2-dichloroethane, sitting or haning drop vapour diffusion technique, mixing 0.002 ml protein solution and 0.001 ml precipitant solution plus 300-600 nl 0.1 M L-proline, optimization of crystallization conditions, enzyme DpcA with 10% v/v 1-bromohexane in a solution of 0.1 M glycine buffer pH 8.6, mixing protein, precipitant solution and 1-bromohexane in a 1:1:1.3 ml ratio and adding 0.00033 ml 0.1 M l-proline as an additive, DpcA complex with 1,2-dichloroethane obtained by soaking experiments after the addition of 0.05 ml 1,2-dichloroethane to the reservoir well containing 0.8 ml of the precipitating solution with the crystals and incubation for 7 days, 19°C, X-ray diffraction structure determination and analysis at 1.05-1.65 A resolution	Psychrobacter cryohalolentis

Crystallization (Comment)

Organism

purified enzyme free or in complex with ligand 1,2-dichloroethane, sitting or haning drop vapour diffusion technique, mixing 0.002 ml protein solution and 0.001 ml precipitant solution plus 300-600 nl 0.1 M L-proline or 0.1 M sarcosine, optimization of crystallization conditions, DmxA complex with 1,2-dichloroethane obtained by soaking experiments after the addition of 0.05 ml 1,2-dichloroethane to the reservoir well containing 0.8 ml of the precipitating solution with the crystals and incubation for 7 days, 19°C, X-ray diffraction structure determination and analysis at 2.10-2.49 A resolution

Marinobacter sp.

purified enzyme free or in complex with ligands 1-bromohexane and 1,2-dichloroethane, sitting or haning drop vapour diffusion technique, mixing 0.002 ml protein solution and 0.001 ml precipitant solution plus 300-600 nl 0.1 M L-proline, optimization of crystallization conditions, enzyme DpcA with 10% v/v 1-bromohexane in a solution of 0.1 M glycine buffer pH 8.6, mixing protein, precipitant solution and 1-bromohexane in a 1:1:1.3 ml ratio and adding 0.00033 ml 0.1 M l-proline as an additive, DpcA complex with 1,2-dichloroethane obtained by soaking experiments after the addition of 0.05 ml 1,2-dichloroethane to the reservoir well containing 0.8 ml of the precipitating solution with the crystals and incubation for 7 days, 19°C, X-ray diffraction structure determination and analysis at 1.05-1.65 A resolution

Psychrobacter cryohalolentis

Organism

Organism	UniProt	Comment	Textmining
Marinobacter sp.	-	gene dmxA	-
Psychrobacter cryohalolentis	-	gene dpcA	-
Psychrobacter cryohalolentis K5	-	gene dpcA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Marinobacter sp.
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Psychrobacter cryohalolentis

Synonyms

Synonyms	Comment	Organism
DmxA	-	Marinobacter sp.
DpcA	-	Psychrobacter cryohalolentis
HLD	-	Marinobacter sp.
HLD	-	Psychrobacter cryohalolentis

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the superfamily of alpha/beta-hydrolases	Marinobacter sp.
evolution	the enzyme belongs to the superfamily of alpha/beta-hydrolases	Psychrobacter cryohalolentis