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3.8.1.5: haloalkane dehalogenase

This is an abbreviated version!
For detailed information about haloalkane dehalogenase, go to the full flat file.

Word Map on EC 3.8.1.5

Reaction

1-Haloalkane
+
H2O
=
a primary alcohol
+
halide

Synonyms

1,3,4,6,-tetrachloro-1,4-cyclohexadiene halidohydrolase, 1-chlorohexane halidohydrolase, 1-haloalkane dehalogenase, DadB, DatA, DbeA, DbjA, DccA, DhaA, DhaA31, DhaB, DhaC, DhAf, DhlA, DhmA, DmaA, dmbA, DmbB, DmbC, dmlA, DmmA, DmrA, DmrB, DmsA, DmtA, DmxA, DpcA, DppA, DrbA, DsaA, DspA, EC 3.8.1.1, eHLD-B, eHLD-C, haloalkane dehalogenase, haloalkane dehalogenase LinB, HanR, HLD, HLD-I, LinB, LinBMI, LinBUT, metallo-haloalkane dehalogenase, protein XP_504164, Rv2579, Ylehd

ECTree

     3 Hydrolases
         3.8 Acting on halide bonds
             3.8.1 In carbon-halide compounds
                3.8.1.5 haloalkane dehalogenase

Purification

Purification on EC 3.8.1.5 - haloalkane dehalogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
five mini proteins are designed via computer modeling and analysis that mimick the main function of haloalkane dehalogenase. A mini protein with 86 amino acids is chosen for His-tag purification
gene dspA, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
-
GJ10 mutant H289Q
-
HiTrap Ni-chelating affinity resin chromatography and Superdex 75 gel filtration
metallo-affinity chromatography
Ni-NTA agarose column chromatography and Superdex 75 gel filtration
Ni-NTA agarose column chromatography, UNO Q6 anion exchange column chromatography and Superdex 75 gel filtration
Ni-NTA column chromatography
Ni-NTA Sepharose column chromatography
-
Ni-NTA-Sepharose column chromatography
recombinant C-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant C-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, anion exchange chromatography and gel filtration
recombinant enzyme 28.1fold from Escherichia coli strain BL21(DE3) by ultrafiltration, anion exchange chromatography, and gel filtration
recombinant enzyme expressed in Pichia pastoris
P51698
recombinant enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged protein
-
recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by metal affinity chromatography
recombinant His6-tagged LinB from Escherichia coli strain BL21 AI by nickel affinity chromatography
-
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3) Rosetta2 by nickel affinity chromatography, ultrafiltration, and gel filtration
-
recombinant N-terminally His-tagged enzyme from Escherichia coli TOP10 by nickel affinity chromatography
recombinant protein from Escherichia coli
recombinant wild-tye and mutant enzymes from Escherichia coli strain Rosetta (DE3)
recombinant wild-type and mutant enzymes from Escherichia coli
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
strain HA1
-
strain m15-3
-
the cell free extract of Rhodococcus erythropolis IAM1399 cells is purified on a BD TALON metal affinity resin
using a glutathione S-transferase (GST)-glutathione affinity column chromatography kit, to remove the GST tag the protein is incubated with factor Xa
using a glutathione sepharose column and size-exclusion chromatography, the GST tag is removed by digestion with AcTEV protease
using a Ni Chelating Sepharose Fast Flow column
-
using TALON metal affinity resin
UT26, His-tagged protein purified on Ni-NTA resin
-