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Literature summary for 3.8.1.5 extracted from

  • Carlucci, L.; Zhou, E.; Malashkevich, V.N.; Almo, S.C.; Mundorff, E.C.
    Biochemical characterization of two haloalkane dehalogenases DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea (2016), Protein Sci., 25, 877-886 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Caulobacter vibrioides
expressed in Escherichia coli BL21(DE3) cells Saccharomonospora azurea
expression in Escherichia coli Caulobacter vibrioides

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, 1.5 A resolution Caulobacter vibrioides
sitting drop vapor diffusion method, using 0.005 M cobalt chloride, 0.005 M magnesium chloride, 0.005 M cadmium chloride, 0.005 M nickel chloride, 0.1M HEPES-NaOH, pH 7.5, 12% (w/v) PEG 3350 Caulobacter vibrioides
sitting drop vapor diffusion method, using 0.005 M cobalt chloride, 0.005 M magnesium chloride, 0.005 M cadmium chloride, 0.005 M nickel chloride, 0.1M HEPES-NaOH, pH 7.5, 12% (w/v) PEG 3350 Saccharomonospora azurea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 1-bromohexane pH 8.2, 25°C Caulobacter vibrioides
1.1
-
 1-bromohexane at pH 8.2 and 25°C Caulobacter vibrioides
1.2
-
 1-Chlorohexane pH 8.2, 25°C Caulobacter vibrioides
1.2
-
 1,5-Dichloropentane pH 8.2, 25°C Caulobacter vibrioides
1.2
-
 1-Chlorohexane at pH 8.2 and 25°C Caulobacter vibrioides
1.2
-
 1,5-Dichloropentane at pH 8.2 and 25°C Caulobacter vibrioides
6.6
-
 1-bromobutane pH 8.2, 25°C Caulobacter vibrioides
6.6
-
 1-bromobutane at pH 8.2 and 25°C Caulobacter vibrioides
7.2
-
 1,3-dibromopropane pH 8.2, 25°C Caulobacter vibrioides
7.2
-
 1,3-dibromopropane at pH 8.2 and 25°C Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides Q9A919
-
-
Caulobacter vibrioides ATCC 19089 Q9A919
-
-
Saccharomonospora azurea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Caulobacter vibrioides
Ni-NTA-Sepharose column chromatography Caulobacter vibrioides
Ni-NTA-Sepharose column chromatography Saccharomonospora azurea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3-tribromopropane + H2O
-
 Caulobacter vibrioides 2,3-dibromopropanol + bromide
-
 ?
1,2,3-tribromopropane + H2O low activity Caulobacter vibrioides 2,3-dibromopropanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Caulobacter vibrioides 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Saccharomonospora azurea 2-bromoethanol + bromide
-
 ?
1,2-dibromoethane + H2O
-
 Caulobacter vibrioides ATCC 19089 2-bromoethanol + bromide
-
 ?
1,2-dibromopropane + H2O
-
 Caulobacter vibrioides 2-bromopropanol + bromide
-
 ?
1,2-dibromopropane + H2O low activity Caulobacter vibrioides 2-bromopropanol + bromide
-
 ?
1,3-dibromopropane + H2O
-
 Saccharomonospora azurea 3-bromopropanol + bromide
-
 ?
1,3-dibromopropane + H2O high activity Caulobacter vibrioides 3-bromopropanol + bromide
-
 ?
1,3-dibromopropane + H2O high activity Caulobacter vibrioides ATCC 19089 3-bromopropanol + bromide
-
 ?
1,5-dichloropentane + H2O
-
 Caulobacter vibrioides 5-chloropentanol + chloride
-
 ?
1,5-dichloropentane + H2O
-
 Saccharomonospora azurea 5-chloropentanol + chloride
-
 ?
1,5-dichloropentane + H2O low activity Caulobacter vibrioides 5-chloropentanol + chloride
-
 ?
1-bromo-3-chloropropane + H2O
-
 Saccharomonospora azurea 3-chloropropanol + bromide
-
 ?
1-bromo-3-chloropropane + H2O high activity Caulobacter vibrioides 3-chloropropanol + bromide
-
 ?
1-bromobutane + H2O high activity Caulobacter vibrioides 1-butanol + bromide
-
 ?
1-bromobutane + H2O high activity Saccharomonospora azurea 1-butanol + bromide
-
 ?
1-bromobutane + H2O high activity Caulobacter vibrioides ATCC 19089 1-butanol + bromide
-
 ?
1-bromohexane + H2O highest activity Caulobacter vibrioides 1-hexanol + bromide
-
 ?
1-bromohexane + H2O highest activity Saccharomonospora azurea 1-hexanol + bromide
-
 ?
1-bromohexane + H2O the enzyme shows highest activity toward 1-bromohexane Caulobacter vibrioides 1-hexanol + bromide
-
 ?
1-chlorohexane + H2O
-
 Caulobacter vibrioides 1-hexanol + chloride
-
 ?
1-chlorohexane + H2O low activity Caulobacter vibrioides 1-hexanol + chloride
-
 ?
1-iodobutane + H2O
-
 Caulobacter vibrioides 1-butanol + iodide
-
 ?
1-iodobutane + H2O
-
 Saccharomonospora azurea 1-butanol + iodide
-
 ?
1-iodobutane + H2O low activity Caulobacter vibrioides 1-butanol + iodide
-
 ?
1-iodopropane + H2O
-
 Caulobacter vibrioides 1-propanol + iodide
-
 ?
1-iodopropane + H2O
-
 Saccharomonospora azurea 1-propanol + iodide
-
 ?
3-bromo-1-propanol + H2O
-
 Caulobacter vibrioides ?
-
 ?
3-bromo-1-propanol + H2O low activity Caulobacter vibrioides ? + bromide
-
 ?
additional information no activity with 1,2,3-tribromopropane, 3-bromo-1-propanol, 1,2-dibromopropane, 1-chlorohexane, 1-chlorobutane, 1,2-dichloroethane and 1,2-dichloropropane Saccharomonospora azurea ?
-
-
additional information no activity with 1-chlorobutane, 1,2-dichloroethane and 1,2-dichloropropane Caulobacter vibrioides ?
-
-
additional information the enzyme shows high activity with both long brominated and chlorinated alkanes. No activity with: 1-chlorobutane, 1,2-dichloroethane, 1,2-dichloropropane Caulobacter vibrioides ?
-
-
additional information no activity with 1-chlorobutane, 1,2-dichloroethane and 1,2-dichloropropane Caulobacter vibrioides ATCC 19089 ?
-
-
additional information the enzyme shows high activity with both long brominated and chlorinated alkanes. No activity with: 1-chlorobutane, 1,2-dichloroethane, 1,2-dichloropropane Caulobacter vibrioides ATCC 19089 ?
-
-

Synonyms

Synonyms Comment Organism
DccA
-
 Caulobacter vibrioides
DsaA
-
 Saccharomonospora azurea
HLD-I
-
 Caulobacter vibrioides
HLD-I
-
 Saccharomonospora azurea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Caulobacter vibrioides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 1-Chlorohexane pH 8.2, 25°C Caulobacter vibrioides
3.1
-
 1-Chlorohexane at pH 8.2 and 25°C Caulobacter vibrioides
5
-
 1,5-Dichloropentane pH 8.2, 25°C Caulobacter vibrioides
5
-
 1,5-Dichloropentane at pH 8.2 and 25°C Caulobacter vibrioides
13
-
 1-bromobutane pH 8.2, 25°C Caulobacter vibrioides
13
-
 1-bromobutane at pH 8.2 and 25°C Caulobacter vibrioides
23.6
-
 1-bromohexane pH 8.2, 25°C Caulobacter vibrioides
23.6
-
 1-bromohexane at pH 8.2 and 25°C Caulobacter vibrioides
29
-
 1,3-dibromopropane pH 8.2, 25°C Caulobacter vibrioides
29
-
 1,3-dibromopropane at pH 8.2 and 25°C Caulobacter vibrioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Caulobacter vibrioides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
 1-bromobutane pH 8.2, 25°C Caulobacter vibrioides
2
-
 1-bromobutane at pH 8.2 and 25°C Caulobacter vibrioides
2.6
-
 1-Chlorohexane pH 8.2, 25°C Caulobacter vibrioides
2.6
-
 1-Chlorohexane at pH 8.2 and 25°C Caulobacter vibrioides
4
-
 1,3-dibromopropane pH 8.2, 25°C Caulobacter vibrioides
4
-
 1,3-dibromopropane at pH 8.2 and 25°C Caulobacter vibrioides
4.2
-
 1,5-Dichloropentane pH 8.2, 25°C Caulobacter vibrioides
4.2
-
 1,5-Dichloropentane at pH 8.2 and 25°C Caulobacter vibrioides
21
-
 1-bromohexane pH 8.2, 25°C Caulobacter vibrioides
21
-
 1-bromohexane at pH 8.2 and 25°C Caulobacter vibrioides