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Literature summary for 3.8.1.5 extracted from

  • Bohac, M.; Nagata, Y.; Prokop, M.; Monincova, M.; Tsuda, M.; Koca, J.; Damborsky, J.
    Halide-stabilizing residues of haloalkane dehalogenases studied by quantum mechanic calculations and site-directed mutagenesis (2002), Biochemistry, 41, 14272-14280.
    View publication on PubMed
Search for more literature for 3.8.1.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli JM109 Sphingomonas paucimobilis

Protein Variants

Protein Variants Comment Organism
F151L site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
F151W site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
F151Y site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
F169L site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
N38D site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
N38E site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
N38F site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
N38Q site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis
W109L site-directed mutagenesis, altered catalytic properties compared to the wild-type enzyme Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of recombinant wild-type and mutant enzymes, analysis of electrostatic interaction energies Sphingomonas paucimobilis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
 strains Y2, m15-3, HA1, GJ70, NCIMB13064, and TB2, enzyme DhaA
-
Sphingomonas paucimobilis
-
 enzyme LinB
-
Sphingomonas paucimobilis UT26
-
 enzyme LinB
-
Xanthobacter autotrophicus P22643 enzyme DhlA
-
Xanthobacter autotrophicus GJ10 P22643 enzyme DhlA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Sphingomonas paucimobilis

Reaction

Reaction Comment Organism Reaction ID
1-haloalkane + H2O = a primary alcohol + halide 3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn38 is involved, SN2 reaction mechanism, modeling, overview Sphingomonas paucimobilis
a
1-haloalkane + H2O = a primary alcohol + halide 3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Asn41 is involved, SN2 reaction mechanism, modeling, overview Rhodococcus erythropolis
a
1-haloalkane + H2O = a primary alcohol + halide 3 features are important for catalytic performance: i. a catalytic triad, ii. anoxyanion hole, and iii. the halide-stabilizing residues, which are not conserved among different haloalkane dehalogenases, active site structure, Glu56 is involved, SN2 reaction mechanism, modeling, overview Xanthobacter autotrophicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chlorobutane + H2O
-
 Rhodococcus erythropolis n-butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Sphingomonas paucimobilis n-butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Xanthobacter autotrophicus n-butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Sphingomonas paucimobilis UT26 n-butanol + chloride
-
 ?
1-chlorobutane + H2O
-
 Xanthobacter autotrophicus GJ10 n-butanol + chloride
-
 ?
1-haloalkane + H2O
-
 Rhodococcus erythropolis primary alcohol + halide
-
 ?
1-haloalkane + H2O
-
 Sphingomonas paucimobilis primary alcohol + halide
-
 ?
1-haloalkane + H2O
-
 Xanthobacter autotrophicus primary alcohol + halide
-
 ?
1-haloalkane + H2O
-
 Sphingomonas paucimobilis UT26 primary alcohol + halide
-
 ?
1-haloalkane + H2O
-
 Xanthobacter autotrophicus GJ10 primary alcohol + halide
-
 ?

Subunits

Subunits Comment Organism
More primary halide-stabilizing residues are Asn38 and Trp109, secondary halide-stabilizing residues are Trp207, Pro208, and Ile211 Rhodococcus erythropolis
More primary halide-stabilizing residues are Asn41 and Trp107, secondary halide-stabilizing residues are Phe205, Pro206, and Ile209 Sphingomonas paucimobilis
More primary halide-stabilizing residues are Trp125 and Trp175, secondary halide-stabilizing residues are Phe172, Pro223, and Val226 Xanthobacter autotrophicus

Synonyms

Synonyms Comment Organism
DhaA
-
 Rhodococcus erythropolis
DhlA
-
 Xanthobacter autotrophicus
LinB
-
 Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sphingomonas paucimobilis