3.8.1.5: haloalkane dehalogenase

This is an abbreviated version!
For detailed information about haloalkane dehalogenase, go to the full flat file.

Reaction

Synonyms

1,3,4,6,-tetrachloro-1,4-cyclohexadiene halidohydrolase, 1-chlorohexane halidohydrolase, 1-haloalkane dehalogenase, DadB, DatA, DbeA, DbjA, DccA, DhaA, DhaA31, DhaB, DhaC, DhAf, DhlA, DhmA, DmaA, dmbA, DmbB, DmbC, dmlA, DmmA, DmrA, DmrB, DmsA, DmtA, DmxA, DpcA, DppA, DrbA, DsaA, DspA, EC 3.8.1.1, eHLD-B, eHLD-C, haloalkane dehalogenase, haloalkane dehalogenase LinB, HanR, HLD, HLD-I, LinB, LinBMI, LinBUT, metallo-haloalkane dehalogenase, protein XP_504164, Rv2579, Ylehd

ECTree

     3 Hydrolases

         3.8 Acting on halide bonds

             3.8.1 In carbon-halide compounds

                3.8.1.5 haloalkane dehalogenase

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Results	in table
4	Activating Compound
4440	AA Sequence
70	Application
1	CAS Registry Number
99	Cloned(Commentary)
51	Crystallization (Commentary)
8	Expression
136	General Information
4	General Stability
60	Inhibitors
62	kcat/KM [mM/s]
5	Ki Value [mM]
159	KM Value [mM]
4	Localization
2	Metals/Ions
28	Molecular Weight [Da]
64	Natural Substrates/ Products (Substrates)
21	Organic Solvent Stability
939	Organism
6	Pathway
148	PDB ID
57	pH Optimum
9	pH Range
4	pH Stability
2	pI Value
2	Posttranslational Modification
152	Protein Variants
57	Purification (Commentary)
51	Reaction
31	Reaction Type
288	Reference
2	Source Tissue
158	Specific Activity [micromol/min/mg]
1	Storage Stability
947	Substrates and Products (Substrate)
72	Subunits
323	Synonyms
1	Systematic Name
50	Temperature Optimum [°C]
4	Temperature Range [°C]
19	Temperature Stability [°C]
101	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

3.6

1,2,3-tribromopropane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

0.005 - 0.24

1,2,3-trichloropropane

3 entries

0.38 - 26.4

1,2-Dibromoethane

5 entries

0.96 - 2.3

1,2-dibromopropane

3 entries

1.05 - 2.37

1,2-Dichloroethane

2 entries

0.91

1,2-dichloropropane

Sphingomonas paucimobilis

-

pH 8.6, 37°C, recombinant enzyme

654645

1.3 - 29

1,3-dibromopropane

13 entries

0.008 - 1.3

1,3-Dichloropropane

4 entries

0.72 - 1.2

1,3-diiodopropane

3 entries

2.45 - 5

1,5-Dichloropentane

3 entries

1.6

1-bromo-2-methylpropane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

0.71 - 13

1-bromobutane

7 entries

0.1

1-bromodecane

Yarrowia lipolytica

Q6C598

pH 4.5, 30°C

758396

0.16

1-bromododecane

Yarrowia lipolytica

Q6C598

pH 4.5, 30°C

758396

1.39

1-bromoheptane

Plesiocystis pacifica

A6G7B1

pH 8.2, 35°C

718667

0.46 - 23.6

1-bromohexane

7 entries

9.94

1-bromopentane

Plesiocystis pacifica

A6G7B1

pH 8.2, 35°C

718667

1.57 - 5.85

1-Bromopropane

3 entries

0.08 - 1.4

1-Chlorobutane

5 entries

3 - 6

1-Chloroheptane

2 entries

0.039 - 6.08

1-Chlorohexane

8 entries

2.75

1-Chlorooctane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

0.48

1-Chloropropane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

0.21

1-Iodobutane

Lyngbya majuscula

Q6DND9

37°C, pH 8.6

755854

0.26 - 2.33

1-iodohexane

2 entries

0.43

1-Iodopropane

Lyngbya majuscula

Q6DND9

37°C, pH 8.6

755854

20.06

2,3-dichloroprop-1-ene

Alcanivorax dieselolei

K0CAI2

pH 8.6, 37°C, recombinant enzyme

735137

3

2-bromoethanol

Rhodococcus rhodochrous

-

above, pH 9.4, 30°C

654715

3

3-bromo-1-propanol

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

8.17

3-chloro-2-(chloromethyl)-1-propene

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

34.26

3-chloro-2-methylprop-1-ene

Alcanivorax dieselolei

K0CAI2

pH 8.6, 37°C, recombinant enzyme

735137

3.5

3-chloro-2-methylpropene

Bradyrhizobium japonicum

-

-

667258

1.2 - 30.8

4-bromobutyronitrile

4 entries

0.05 - 0.11

bis(2-chloroethyl) ether

3 entries

0.59 - 1.36

bromocyclohexane

2 entries

0.25

chlorocyclohexane

Bradyrhizobium japonicum

-

-

667258

additional information

additional information

4 entries

-

0.005

1,2,3-trichloropropane

Sphingobium indicum

A8CFB7

-

684553

0.08

1,2,3-trichloropropane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

0.24

1,2,3-trichloropropane

Bradyrhizobium japonicum

-

-

667258

0.38

1,2-Dibromoethane

Plesiocystis pacifica

A6G7B1

pH 8.2, 35°C

718667

1.8

1,2-Dibromoethane

Lyngbya majuscula

Q6DND9

37°C, pH 8.6

755854

14.3

1,2-Dibromoethane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

17.95

1,2-Dibromoethane

Alcanivorax dieselolei

K0CAI2

pH 8.6, 37°C, recombinant enzyme

735137

26.4

1,2-Dibromoethane

Sphingomonas paucimobilis

-

pH 8.6, 37°C, recombinant wild-type enzyme, data from 2 experimental sets

656190

0.96

1,2-dibromopropane

Bradyrhizobium japonicum

-

-

667258

1.97

1,2-dibromopropane

Plesiocystis pacifica

A6G7B1

pH 8.2, 35°C

718667

2.3

1,2-dibromopropane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

1.05

1,2-Dichloroethane

Sphingomonas paucimobilis

-

pH 8.6, 37°C, recombinant enzyme

654645

2.37

1,2-Dichloroethane

Ancylobacter aquaticus

B8YPW8

pH 9.0, 37°C, recombinant enzyme

735217

1.3

1,3-dibromopropane

Agrobacterium tumefaciens

E2RV69

recombinant mutant Y109W, pH 8.6, 37°C

733827

1.47

1,3-dibromopropane

Agrobacterium tumefaciens

E2RV69

pH 8.6, 37°C, DatA

718635

1.5

1,3-dibromopropane

Agrobacterium tumefaciens

E2RV69

recombinant wild-type enzyme, pH 8.6, 37°C

733827

1.57

1,3-dibromopropane

Strongylocentrotus purpuratus

-

pH 8.6, 37°C, recombinant His-tagged enzyme

733432

2.4

1,3-dibromopropane

uncultured marine bacterium

-

pH and temperature not specified in the publication

720997

2.7

1,3-dibromopropane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

2.72

1,3-dibromopropane

Psychrobacter cryohalolentis

-

pH 8.7, 37°C

718650

3.6

1,3-dibromopropane

Bradyrhizobium japonicum

-

-

667258

6.6

1,3-dibromopropane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

9.2

1,3-dibromopropane

Mycobacterium tuberculosis variant bovis

Q9XB14

pH 8.5

667261

13.39

1,3-dibromopropane

Alcanivorax dieselolei

K0CAI2

pH 8.6, 37°C, recombinant enzyme

735137

29

1,3-dibromopropane

Caulobacter vibrioides

Q9A919

pH 8.2, 25°C

758291

29

1,3-dibromopropane

Caulobacter vibrioides

Q9A919

at pH 8.2 and 25°C

758291

0.008

1,3-Dichloropropane

Agrobacterium tumefaciens

E2RV69

pH 8.6, 37°C, DatA

718635

0.01

1,3-Dichloropropane

Agrobacterium tumefaciens

E2RV69

below, recombinant wild-type enzyme, pH 8.6, 37°C

733827

0.012

1,3-Dichloropropane

Agrobacterium tumefaciens

E2RV69

below, recombinant mutant Y109W, pH 8.6, 37°C

733827

1.3

1,3-Dichloropropane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

0.72

1,3-diiodopropane

Agrobacterium tumefaciens

E2RV69

recombinant mutant Y109W, pH 8.6, 37°C

733827

1.2

1,3-diiodopropane

Agrobacterium tumefaciens

E2RV69

pH 8.6, 37°C, DatA

718635

1.2

1,3-diiodopropane

Agrobacterium tumefaciens

E2RV69

recombinant wild-type enzyme, pH 8.6, 37°C

733827

2.45

1,5-Dichloropentane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

5

1,5-Dichloropentane

Caulobacter vibrioides

Q9A919

pH 8.2, 25°C

758291

5

1,5-Dichloropentane

Caulobacter vibrioides

Q9A919

at pH 8.2 and 25°C

758291

0.71

1-bromobutane

Agrobacterium tumefaciens

E2RV69

recombinant mutant Y109W, pH 8.6, 37°C

733827

1.1

1-bromobutane

Psychrobacter cryohalolentis

-

pH 8.7, 37°C

718650

1.5

1-bromobutane

Agrobacterium tumefaciens

E2RV69

recombinant wild-type enzyme, pH 8.6, 37°C

733827

3.41

1-bromobutane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

8.08

1-bromobutane

Plesiocystis pacifica

A6G7B1

pH 8.2, 35°C

718667

13

1-bromobutane

Caulobacter vibrioides

Q9A919

pH 8.2, 25°C

758291

13

1-bromobutane

Caulobacter vibrioides

Q9A919

at pH 8.2 and 25°C

758291

0.46

1-bromohexane

Agrobacterium tumefaciens

E2RV69

recombinant mutant Y109W, pH 8.6, 37°C

733827

1.05

1-bromohexane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

1.7

1-bromohexane

Agrobacterium tumefaciens

E2RV69

pH 8.6, 37°C, DatA

718635

1.7

1-bromohexane

Agrobacterium tumefaciens

E2RV69

recombinant wild-type enzyme, pH 8.6, 37°C

733827

2.69

1-bromohexane

Plesiocystis pacifica

A6G7B1

pH 8.2, 35°C

718667

23.6

1-bromohexane

Caulobacter vibrioides

Q9A919

pH 8.2, 25°C

758291

23.6

1-bromohexane

Caulobacter vibrioides

Q9A919

at pH 8.2 and 25°C

758291

1.57

1-Bromopropane

Yarrowia lipolytica

Q6C598

pH 4.5, 30°C

758396

2.7

1-Bromopropane

Rhodococcus rhodochrous

-

pH 9.4, 30°C

654715

5.85

1-Bromopropane

Plesiocystis pacifica

A6G7B1

pH 8.2, 35°C

718667

0.08

1-Chlorobutane

Mycobacterium tuberculosis variant bovis

Q9XB14

pH 8.5

667261

0.17

1-Chlorobutane

Lyngbya majuscula

Q6DND9

37°C, pH 8.6

755854

1.055

1-Chlorobutane

Sphingomonas paucimobilis

-

pH 8.6, 37°C, recombinant wild-type enzyme, data from 2 experimental sets

656190

1.17

1-Chlorobutane

Alcanivorax dieselolei

K0CAI2

pH 8.6, 37°C, recombinant enzyme

735137

1.4

1-Chlorobutane

Bradyrhizobium japonicum

-

-

667258

3 - 6

1-Chloroheptane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

3.345

1-Chloroheptane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

0.039

1-Chlorohexane

Agrobacterium tumefaciens

E2RV69

pH 8.6, 37°C, DatA

718635

0.04

1-Chlorohexane

Agrobacterium tumefaciens

E2RV69

recombinant mutant Y109W, pH 8.6, 37°C

733827

0.07

1-Chlorohexane

Agrobacterium tumefaciens

E2RV69

recombinant wild-type enzyme, pH 8.6, 37°C

733827

1.033

1-Chlorohexane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

1.4

1-Chlorohexane

Bradyrhizobium japonicum

-

-

667258

3.1

1-Chlorohexane

Caulobacter vibrioides

Q9A919

pH 8.2, 25°C

758291

3.1

1-Chlorohexane

Caulobacter vibrioides

Q9A919

at pH 8.2 and 25°C

758291

6.08

1-Chlorohexane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

0.26

1-iodohexane

Agrobacterium tumefaciens

E2RV69

pH 8.6, 37°C, DatA

718635

2.33

1-iodohexane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

1.2

4-bromobutyronitrile

Lyngbya majuscula

Q6DND9

37°C, pH 8.6

755854

3.1

4-bromobutyronitrile

Mycolicibacterium rhodesiae

G4I2J6, G4I5P8

pH 8.0, 25°C, recombinant enzyme

734726

3.1

4-bromobutyronitrile

Mycolicibacterium rhodesiae

G4I2J6, G4I5P8

at pH 8.0 and 25°C

757696

30.8

4-bromobutyronitrile

Alcanivorax dieselolei

K0CAI2

pH 8.6, 37°C, recombinant enzyme

735137

0.05

bis(2-chloroethyl) ether

Rhodococcus rhodochrous

P0A3G2

37°C, pH 8.2, enzyme multi-PEGylated with the thiosuccinimido butylamine linker

757271

0.08

bis(2-chloroethyl) ether

Rhodococcus rhodochrous

P0A3G2

37°C, pH 8.2, enzyme mono-PEGylated with the thiosuccinimido butylamine linker

757271

0.11

bis(2-chloroethyl) ether

Rhodococcus rhodochrous

P0A3G2

37°C, pH 8.2, wild-type enzyme

757271

0.59

bromocyclohexane

Bradyrhizobium japonicum

-

-

667258

1.36

bromocyclohexane

Sphingomonas paucimobilis

-

pH 8.6, 37°C

667656

additional information

additional information

Sphingomonas paucimobilis

-

-

656190

-

additional information

additional information

Xanthobacter autotrophicus

P22643

rate limiting reaction step is the release of halide

247097

-

additional information

additional information

Xanthobacter autotrophicus

-

GJ10, rate limiting step is hydrolysis of alkyl-enzyme intermediate

247089

-

additional information

additional information

Rhodococcus rhodochrous

-

rates of reaction steps

654715

-