3.5.4.33: tRNA(adenine34) deaminase
This is an abbreviated version!
For detailed information about tRNA(adenine34) deaminase, go to the full flat file.
Word Map on EC 3.5.4.33
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3.5.4.33
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deamination
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wobble
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anticodons
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heterodimeric
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a-to-i
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deaminases
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chloroplast
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decode
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intellectual
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trna-specific
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disability
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trypanosoma
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trnaarg
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trypanosomatids
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adats
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base-pairing
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cytidine-to-uridine
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post-transcriptionally
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brucei
- 3.5.4.33
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deamination
-
wobble
-
anticodons
-
heterodimeric
-
a-to-i
- deaminases
- chloroplast
-
decode
-
intellectual
-
trna-specific
-
disability
-
trypanosoma
- trnaarg
-
trypanosomatids
-
adats
-
base-pairing
-
cytidine-to-uridine
-
post-transcriptionally
- brucei
Reaction
Synonyms
ADAT2-ADAT3 complex, ADAT2/3, adenosine-to-inosine tRNA-editing enzyme, AtTadA, spTAD, spTadA, TadA, tadA protein, tadA/ecADAT2, tRNA A:34 deaminase, tRNA adenosine deaminase arginine, tRNA:A34 deaminase, tRNA:adenosine 34 deaminase
ECTree
3.5.4.33 tRNA(adenine34) deaminaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.5.4.33 - tRNA(adenine34) deaminase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
adenine34 in tRNAVal + H2O
hypoxanthine34 in tRNAVal + NH3
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-
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
P47058; Q9URQ3
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
P47058; Q9URQ3
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
P47058; Q9URQ3
enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
P47058; Q9URQ3
the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the chloroplast tRNA editing protein is specifically required for deamination of chloroplast (cp)-tRNAArg(ACG) to cp-tRNAArg(ICG)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
complementation studies show that the C-terminal domain is sufficient for cognate tRNA deamination both in vitro and in planta. TADA is exclusively involved in editing of the prokaryote-type cp-tRNAArg(ACG). No editing of cytosolic tRNAAla(AGC), tRNAVal(AAC), and tRNAThr(AGU). TADA is also not involved in editing of nucleotide A37 of tRNAAla(AGC)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
-
kinetic isotope effects and quantum molecular modeling are applied to solve the transition state structure of the enzyme. The key events of the tRNA editing process are facilitated by the enzyme-promoted base flip and the Glu70-mediated proton shuttle
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. With the exception of yeast tRNAArg, no other eukaryotic tRNA substrates are found to be modified by tadA. An artificial yeast tRNAAsp, which carries the anticodon loop of yeast tRNAArg, is bound and modified by tadA. A tRNAArg2 minisubstrate containing the anticodon stem and loop is sufficient for specific deamination by tadA. Nucleotides at positions 33-36 are sufficient for inosine formation in mutant Arg2 minisubstrates. The anticodon is thus a major determinant for tadA substrate specificity
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
P47058; Q9URQ3
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
P47058; Q9URQ3
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
-
-
?
adenine34 in tRNAArg2 + H2O
hypoxanthine34 in tRNAArg2 + NH3
the bacterial tRNA adenosine deaminase generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system
-
-
?
adenine34 in tRNAThr(AGU) + H2O
hypoxanthine34 in tRNAThr(IGU) + NH3
-
-
-
-
?
adenine34 in tRNAThr(AGU) + H2O
hypoxanthine34 in tRNAThr(IGU) + NH3
-
in vitro, this enzyme can mediate A-to-I editing of tRNA and C-to-U deamination of ssDNA
-
-
?
