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3.5.4.33: tRNA(adenine34) deaminase

This is an abbreviated version!
For detailed information about tRNA(adenine34) deaminase, go to the full flat file.

Word Map on EC 3.5.4.33

Reaction

adenine34 in tRNA
+
H2O
=
hypoxanthine34 in tRNA
 +
NH3

Synonyms

ADAT2-ADAT3 complex, ADAT2/3, adenosine-to-inosine tRNA-editing enzyme, AtTadA, spTAD, spTadA, TadA, tadA protein, tadA/ecADAT2, tRNA A:34 deaminase, tRNA adenosine deaminase arginine, tRNA:A34 deaminase, tRNA:adenosine 34 deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.33 tRNA(adenine34) deaminase

Engineering

Engineering on EC 3.5.4.33 - tRNA(adenine34) deaminase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F144A
mutations results in a reduction in enzymatic activity of about 800-fold
F145A
inactive mutant enzyme
R149A
mutations results in a reduction in enzymatic activity of about 800-fold
R94A
mutation substantially 220fold reduces the enzymatic activity
D64E
D64E abolishes the enzymatic activity of tadA in vitro, it does not detectably affect its activity in cells
C-terDELTA10
-
mutation in subunit ADAT2, inactive mutant enzyme
C-terDELTA5
-
mutation in subunit ADAT2, kcat/Km is 72% compared to the wild-type value
C136A
-
kcat/Km is 13% of the wild-type value, mutation in subunit ADAT2
C136A/ADAT3 C291A
-
inactive mutant enzyme, mutation in subunit ADAT2
C139A
-
inactive mutant enzyme, mutation in subunit ADAT2
C291A
-
inactive mutant enzyme, mutation in subunit ADAT3
C294A
-
inactive mutant enzyme, mutation in subunit ADAT3
E92A/K216A/R217A/K218A/R219A
-
inactive mutant enzyme
H252A
-
inactive mutant enzyme, mutation in subunit ADAT3
H90A
-
inactive mutant enzyme, mutation in subunit ADAT2
K216A
-
mutation in subunit ADAT2, kcat/Km is 12% compared to the wild-type value
K218A
-
mutation in subunit ADAT2, kcat/Km is 24% compared to the wild-type value
R217A
-
mutation in subunit ADAT2, kcat/Km is 16% compared to the wild-type value
R219A
-
mutation in subunit ADAT2, kcat/Km is 20% compared to the wild-type value
V254E
-
inactive mutant enzyme, mutation in subunit ADAT3
V254L
-
kcat/Km is 7% of the wild-type value, mutation in subunit ADAT3
V254T
-
kcat/Km is 20% of the wild-type value, mutation in subunit ADAT3
additional information
-
deletion of the last 10 amino acids at the C terminus of TbADAT2 abolishes tRNA binding. In addition, single alanine replacements of a string of positively charged amino acids (KRKRK) lead to binding defects that correlate with losses in enzyme activity