Any feedback?
Literature summary for 3.5.4.33 extracted from
- Biochemical and structural studies of A-to-I editing by tRNA:A34 deaminases at the wobble position of transfer RNA (2005), Biochemistry, 44, 12057-12065.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichi coli | Escherichia coli |
| overexpression in Escherichi coli | Aquifex aeolicus |
| overexpression in Escherichia coli | Agrobacterium tumefaciens |
| overexpression in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| tadA missing the last eight amino acids at the C-terminus is produced high-quality crystals, and the structure is determined at 1.6 A resolution, hanging drop vapor diffusion method | Agrobacterium tumefaciens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F144A | mutations results in a reduction in enzymatic activity of about 800-fold | Agrobacterium tumefaciens |
| F145A | inactive mutant enzyme | Agrobacterium tumefaciens |
| R149A | mutations results in a reduction in enzymatic activity of about 800-fold | Agrobacterium tumefaciens |
| R94A | mutation substantially 220fold reduces the enzymatic activity | Agrobacterium tumefaciens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | the active site contains a zinc ion, which is coordinated by three strictly conserved amino acids, His53, Cys83, and Cys86 | Agrobacterium tumefaciens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine34 in tRNAArg + H2O | Escherichia coli | - |
hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Aquifex aeolicus | - |
hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Agrobacterium tumefaciens | - |
hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Saccharomyces cerevisiae | - |
hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Saccharomyces cerevisiae ATCC 204508 | - |
hypoxanthine34 in tRNAArg + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | A9CK16 | - |
- |
| Aquifex aeolicus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Saccharomyces cerevisiae | P47058 and Q9URQ3 | P47058: TAD2 and Q9URQ3: TAD3 | - |
| Saccharomyces cerevisiae ATCC 204508 | P47058 and Q9URQ3 | P47058: TAD2 and Q9URQ3: TAD3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Aquifex aeolicus |
- |
Agrobacterium tumefaciens |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine34 in tRNAArg + H2O | - |
Escherichia coli | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | - |
Aquifex aeolicus | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | - |
Agrobacterium tumefaciens | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | - |
Saccharomyces cerevisiae | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) | Escherichia coli | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) | Aquifex aeolicus | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) | Agrobacterium tumefaciens | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) | Saccharomyces cerevisiae | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
| adenine34 in tRNAArg + H2O | Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA) | Saccharomyces cerevisiae ATCC 204508 | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Escherichia coli |
| homodimer | - |
Aquifex aeolicus |
| homodimer | - |
Agrobacterium tumefaciens |
| homodimer | - |
Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADAT2-ADAT3 complex | - |
Saccharomyces cerevisiae |
| tadA protein | - |
Escherichia coli |
| tadA protein | - |
Aquifex aeolicus |
| tadA protein | - |
Agrobacterium tumefaciens |
| tRNA:A34 deaminase | - |
Escherichia coli |
| tRNA:A34 deaminase | - |
Aquifex aeolicus |
| tRNA:A34 deaminase | - |
Agrobacterium tumefaciens |
| tRNA:A34 deaminase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Aquifex aeolicus |
| 37 | - |
assay at | Agrobacterium tumefaciens |
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Escherichia coli |
| 6 | - |
assay at | Aquifex aeolicus |
| 6 | - |
assay at | Agrobacterium tumefaciens |
| 6 | - |
assay at | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
