Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.4.33: tRNA(adenine34) deaminase

This is an abbreviated version!
For detailed information about tRNA(adenine34) deaminase, go to the full flat file.

Word Map on EC 3.5.4.33

Reaction

adenine34 in tRNA
+
H2O
=
hypoxanthine34 in tRNA
 +
NH3

Synonyms

ADAT2-ADAT3 complex, ADAT2/3, adenosine-to-inosine tRNA-editing enzyme, AtTadA, spTAD, spTadA, TadA, tadA protein, tadA/ecADAT2, tRNA A:34 deaminase, tRNA adenosine deaminase arginine, tRNA:A34 deaminase, tRNA:adenosine 34 deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.33 tRNA(adenine34) deaminase

Crystallization

Crystallization on EC 3.5.4.33 - tRNA(adenine34) deaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
tadA missing the last eight amino acids at the C-terminus is produced high-quality crystals, and the structure is determined at 1.6 A resolution, hanging drop vapor diffusion method
hanging drop vapor diffusion method, 1.8 A resolution. The large cavity formed around the catalytic site with the zinc ion seems to be important for accommodating the anticodon loop stem of tRNAArg-2
structure determination to 2.0 A of Staphylococcus aureus TadA bound to the anticodon stem-loop of tRNA(Arg2) bearing nebularine, a non-hydrolyzable adenosine analog, at the wobble position
crystal structure determined at 2.0 A resolution by multiwavelength anomalous dispersion using bound Zn2+
crystallization at 22°C by the sitting-drop method, in the presence of Zn2+ ion using ammonium sulfate as a precipitant. Flash-cooled crystals diffract to 2.0 A using 30% (v/v) glycerol as a cryoprotectant, crystal belongs to the tetragonal space group P4(2)2(1)2