Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D64E | D64E abolishes the enzymatic activity of tadA in vitro, it does not detectably affect its activity in cells | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18718 | - |
2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine34 in tRNAArg + H2O | Escherichia coli | the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli | hypoxanthine34 in tRNAArg + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P68398 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine34 in tRNAArg + H2O | the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. tadA is an essential gene in Escherichiy coli | Escherichia coli | hypoxanthine34 in tRNAArg + NH3 | - |
? | |
adenine34 in tRNAArg + H2O | the enzyme catalyzes the site-specific inosine formation at the wobble position (position 34) of tRNAArg2, the only tRNA having this modification in prokaryotes. With the exception of yeast tRNAArg, no other eukaryotic tRNA substrates are found to be modified by tadA. An artificial yeast tRNAAsp, which carries the anticodon loop of yeast tRNAArg, is bound and modified by tadA. A tRNAArg2 minisubstrate containing the anticodon stem and loop is sufficient for specific deamination by tadA. Nucleotides at positions 33-36 are sufficient for inosine formation in mutant Arg2 minisubstrates. The anticodon is thus a major determinant for tadA substrate specificity | Escherichia coli | hypoxanthine34 in tRNAArg + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 18718, can form a homodimer in vitro, it is not proved that it is active as a homodimer in vitro, calculated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TadA | - |
Escherichia coli |
tadA/ecADAT2 | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | disruption of the wild-type chromosomal copy of tadA results in viable Escherichia coli only when an additional plasmid-borne tadA gene is also present. TadA is essential for viability of eubacterial organisms | Escherichia coli |