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Literature summary for 3.5.4.33 extracted from

  • Spears, J.L.; Rubio, M.A.; Gaston, K.W.; Wywial, E.; Strikoudis, A.; Bujnicki, J.M.; Papavasiliou, F.N.; Alfonzo, J.D.
    A single zinc ion is sufficient for an active Trypanosoma brucei tRNA editing deaminase (2011), J. Biol. Chem., 286, 20366-20374.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.33

Protein Variants

Protein Variants Comment Organism
C136A kcat/Km is 13% of the wild-type value, mutation in subunit ADAT2 Trypanosoma brucei
C136A/ADAT3 C291A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
C139A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
C291A inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
C294A inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
E92A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
H252A inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
H90A inactive mutant enzyme, mutation in subunit ADAT2 Trypanosoma brucei
V254E inactive mutant enzyme, mutation in subunit ADAT3 Trypanosoma brucei
V254L kcat/Km is 7% of the wild-type value, mutation in subunit ADAT3 Trypanosoma brucei
V254T kcat/Km is 20% of the wild-type value, mutation in subunit ADAT3 Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00075
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei
0.0015
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2 Trypanosoma brucei
0.0021
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2 Trypanosoma brucei
0.0028
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3 Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ wild type enzyme coordinates two Zn2+ per heterodimer. Mutation of one of the key Zn2+-coordinating cysteines in TbADAT2 yields a functional enzyme with a single bound zinc Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine34 in tRNAVal + H2O Trypanosoma brucei
-
 hypoxanthine34 in tRNAVal + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine34 in tRNAVal + H2O
-
 Trypanosoma brucei hypoxanthine34 in tRNAVal + NH3
-
 ?

Synonyms

Synonyms Comment Organism
ADAT2/3
-
 Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00083
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2 Trypanosoma brucei
0.00083
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3 Trypanosoma brucei
0.0018
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2 Trypanosoma brucei
0.0032
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.3
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254L, mutation in subunit ADAT3 Trypanosoma brucei
0.55
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme C136A, mutation in subunit ADAT2 Trypanosoma brucei
0.86
-
 adenine34 in tRNAVal 27°C, pH 8.0, mutant enzyme V254T, mutation in subunit ADAT2 Trypanosoma brucei
4.3
-
 adenine34 in tRNAVal 27°C, pH 8.0, wild-type enzyme Trypanosoma brucei