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Literature summary for 3.5.4.33 extracted from
- Mechanism, specificity and general properties of the yeast enzyme catalysing the formation of inosine 34 in the anticodon of transfer RNA (1996), J. Mol. Biol., 262, 437-458.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5 mM abolishes activity up to 70% | Saccharomyces cerevisiae |  |
| 4,7-phenanthroline | 5 mM abolishes activity up to 70% | Saccharomyces cerevisiae | |
| additional information | no inhibition by 2'-(8R)-deoxycoformycin up to 12 mM | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000023 | - |
adenine34 in tRNASer(AGA) | pH and temperature not specified in the publication | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimum concentration2-5 mM, the enzyme does not require any cofactor, except magnesium ions, to deaminate adenosine 34 efficiently in tRNA. The dependence of the enzymatic reaction on magnesium ions probably reflects the need for a correct tRNA architecture | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine34 in tRNA + H2O | Saccharomyces cerevisiae | enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast | hypoxanthine34 in tRNA + NH3 | - |
? | |
| adenine34 in tRNA + H2O | Saccharomyces cerevisiae ATCC 204508 | enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast | hypoxanthine34 in tRNA + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P47058 and Q9URQ3 | P47058: TAD2 and Q9URQ3: TAD3 | - |
| Saccharomyces cerevisiae ATCC 204508 | P47058 and Q9URQ3 | P47058: TAD2 and Q9URQ3: TAD3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine34 in tRNA + H2O | enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast | Saccharomyces cerevisiae | hypoxanthine34 in tRNA + NH3 | - |
? | |
| adenine34 in tRNA + H2O | the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast | Saccharomyces cerevisiae | hypoxanthine34 in tRNA + NH3 | - |
? | |
| adenine34 in tRNA + H2O | enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast | Saccharomyces cerevisiae ATCC 204508 | hypoxanthine34 in tRNA + NH3 | - |
? | |
| adenine34 in tRNA + H2O | the efficacy of adenosine 34 to inosine 34 conversion depends on the nucleotide sequence of the anticodon loop and its proximal stem, the best tRNA substrates being those with a purine at position 35. The enzyme accounts for the presence of inosine 34 in all seven cytoplasmic A34-containing precursor tRNAs in yeast | Saccharomyces cerevisiae ATCC 204508 | hypoxanthine34 in tRNA + NH3 | - |
? | |
| adenine34 in tRNAAla(AGC) + H2O | - |
Saccharomyces cerevisiae | hypoxanthine34 in tRNAAla(IGC) + NH3 | - |
? | |
| adenine34 in tRNAAla(AGC) + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | hypoxanthine34 in tRNAAla(IGC) + NH3 | - |
? | |
| adenine34 in tRNASer(AGA) + H2O | - |
Saccharomyces cerevisiae | hypoxanthine34 in tRNASer(IGA) + NH3 | - |
? | |
| adenine34 in tRNASer(AGA) + H2O | - |
Saccharomyces cerevisiae ATCC 204508 | hypoxanthine34 in tRNASer(IGA) + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tRNA:adenosine 34 deaminase | - |
Saccharomyces cerevisiae |
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