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Literature summary for 3.5.4.33 extracted from

  • Kuratani, M.; Ishii, R.; Bessho, Y.; Fukunaga, R.; Sengoku, T.; Shirouzu, M.; Sekine, S.; Yokoyama, S.
    Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus (2005), J. Biol. Chem., 280, 16002-16008.
    View publication on PubMed
Search for more literature for 3.5.4.33

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, 1.8 A resolution. The large cavity formed around the catalytic site with the zinc ion seems to be important for accommodating the anticodon loop stem of tRNAArg-2 Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ TadA contains endogenous Zn2+ ions. Zn2+ is tetrahedrally coordinated by His52, Cys82, Cys85, and a water Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine34 in tRNAArg2 + H2O Aquifex aeolicus the bacterial tRNA adenosine deaminase generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system hypoxanthine34 in tRNAArg2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O67050
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine34 in tRNAArg2 + H2O
-
 Aquifex aeolicus hypoxanthine34 in tRNAArg2 + NH3
-
 ?
adenine34 in tRNAArg2 + H2O the bacterial tRNA adenosine deaminase generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system Aquifex aeolicus hypoxanthine34 in tRNAArg2 + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Aquifex aeolicus

Synonyms

Synonyms Comment Organism
TadA
-
 Aquifex aeolicus