Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.24.B10: ADAM12 endopeptidase

This is an abbreviated version!
For detailed information about ADAM12 endopeptidase, go to the full flat file.

Word Map on EC 3.4.24.B10

Reaction

proteolysis of proteins =

Synonyms

a disintegrin and metalloprotease 12, a disintegrin and metalloprotease with thrombospondin motifs 12, a disintegrin and metalloprotease-12, a disintegrin and metalloproteinase 12, a disintegrin and metalloproteinase domain 12, a disintegrin and metalloproteinase domain-containing protein 12, a disintegrin and metalloproteinase12, ADAM 12, ADAM 12-S, ADAM-12, ADAM-12m, ADAM-PCD, ADAM12, ADAM12-L, ADAM12-PC, ADAM12-S, ADAM12/meltrin alpha, ADAM12L, ADAM12S, ADAMTS-12, ADMA12L, basigin sheddase, disintegrin and metalloprotease 12, full-length ADAM 12-S, M12.212, meltrin alpha, meltrin-alpha, metalloprotease-disintegrin ADAM 12, metalloprotease-disintegrin ADAM12

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B10 ADAM12 endopeptidase

Cloned

Cloned on EC 3.4.24.B10 - ADAM12 endopeptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in 293-VnR cell (derived from HEK-293 cell)
-
expressed in COS-7 cells
-
expressed in COS-7 cells, CHO-K1 cells, NHI-3T3 cells, and MCF-7 cells
-
expressed in Drosophila S2 cells and Escherichia coli
-
expressed in embryonic kidney cell line
-
expressed in HEK-293 cell
expressed in HEK-293T cells, hepatic stellate cells, rhabdomyosarcoma cells, C2C12 cells, COS7 cells, and MvLu1 cells
-
expressed in MCF-7 cells
expression as GFP-pleckstrin homology domain fusion protein in C2C12 cells, targeted to the plasma membrane by a myristoylation motif
-
expression as soluble GST-fusion protein in Escherichia coli, and expression of an enzyme fragment, comprising amino acid residues 743-903 of the cytoplasmic domain, as GFP-fusion protein in C2C12 cells
-
expression in a yeast two-hybrid system as GT-tagged enzyme
-
expression in CHO cells
-
expression of the enzymes cytoplasmic tail including or missing the alpha-actinin-2-binding site, tagged with a myristolation motif in C2C12 myoblast cells, in vitro transcription and translation of the enzyme, expression of the enzymes cytoplasmic tail including or missing the alpha-actinin-2-binding site as GST-fusion proteins in Escherichia coli XL-1 Blue
expression of the soluble enzyme form ADAM 12-S in COS cells
expression of the soluble enzyme in COS-1 cells and in 293-EBNA cell line, coexpression of soluble enzyme and insulin-like growth factor-binding protein-3 in a Saccharomyces cerevisiae two hybrid expression system
expression of the soluble secreted enzyme form ADAM 12-S in cell line 293-EBNA
overexpression in C2C12 cells, leading to upregulation of retinoblastoma-related proteins p130, cell cycle inhibitor protein p27, and the differentiation markers myogenin and integrin alpha7A isoform, all resulting in cell cycle arrest
-
transient expression in COS-7 cells of an enzyme mutant where the signal peptide, prodomain and metalloprotease domain are exchanged for an Ig kappa-chain leader sequence, secretion to the medium
transient expression in COS-7 cells of the enzyme mutants, secretion to the medium