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3.4.22.55: caspase-2

This is an abbreviated version!
For detailed information about caspase-2, go to the full flat file.

Word Map on EC 3.4.22.55

Reaction

strict requirement for an Asp residue at P1, with Asp316 being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-/- =

Synonyms

AjCASP, C14.006, CASP-2, Casp2, caspase 2, caspase-2, caspase-2L, caspase-2S, ICH-1, ICH-1 protease, ICH-1L/1S, NEDD-2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.55 caspase-2

Cloned

Cloned on EC 3.4.22.55 - caspase-2

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
caspase-2 maps to the q34-35 segment of human chromosome 7
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commercial preparation of cDNA, in vitro transcription/translation
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expressed as fusion protein in HeLa cell and HEK-293T cell
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expressed in HEK-293T cells
expressed in Jurkat cells
expression in Saccharomyces cerevisiae and Escherichia coli
expression of procaspase-2 in Escherichia coli strain BL21(DE3)
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expression of wild-type and mutant enzymes in Escherichia coli strain Bl21 (DE3) pLysS
GST-fusion contruct expressed in Escherichia coli BL21
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GST-fusion protein expressed in Escherichia coli BL21
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in vitro translation, His6-tagged protein expressed in Escherichia coli BL21
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wild-type, mutants C303A and D316G are expressed in Escherichia coli