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Literature summary for 3.4.22.55 extracted from

  • Karki, P.; Dahal, G.R.; Shin, S.Y.; Lee, J.S.; Cho, B.; Park, I.S.
    Efficient cleavage of Bid and procaspase-7 by caspase-2 at lower pH (2008), Protein Pept. Lett., 15, 1044-1049.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of procaspase-2 in Escherichia coli strain BL21(DE3) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl activates at 50 mM, the enzyme is stable until 150 mM NaCl Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procaspase-7 + H2O Homo sapiens activation caspase-7 + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant procaspase-2 from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 95% Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
strict requirement for an Asp residue at P1, with Asp316 being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-/- catalysis is mediated by a mechanism involving a catalytic dyad composed of a cysteine residue as the catalytic nucleophile and a histidine residue Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neuroblastoma cell
-
Homo sapiens
-
SK-N-BE(2) cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procaspase-7 + H2O activation Homo sapiens caspase-7 + ?
-
?
procaspase-7 + H2O activation by cleavage at Asp198 and Asp206 Homo sapiens caspase-7 + ?
-
?
VDVAD-7-amido-4-methylcoumarin + H2O
-
Homo sapiens VDVAD + 7-amino-4-methylcoumarin
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.2
-
Homo sapiens