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Literature summary for 3.4.22.55 extracted from

  • Ho, P.K.; Jabbour, A.M.; Ekert, P.G.; Hawkins, C.J.
    Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 (2005), FEBS J., 272, 1401-1414.
    View publication on PubMed
Search for more literature for 3.4.22.55

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae and Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C303A inactive Homo sapiens
D152A pro-caspase mutant, like the wild-type, this mutant is efficiently processed between the large and the small subunit, however, it is not further processed to seperate the prodomain from the large subunit Homo sapiens
D316A fusion of the linker to the large subunit, toxic when expressed in yeast Homo sapiens
D316A/D330A abolishes auto-processing and reduces enzymatic activity dramatically, 840fold decrease in activity Homo sapiens
D330A fusion of the linker to the small subunit, slightly greater deleterious effect on enzyme activity than fusion to the large subunit (D316A), toxic when expressed in yeast Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information caspase-2 is not subject to inhibition by members of the IAP family of apoptosis inhibitors Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P42575
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-DEVD-7-amino-4-trifluoromethyl coumarin + H2O 37°C, pH 7.0, 10 mM dithiothreitol Homo sapiens 7-amino-4-trifluoromethyl coumarin + Ac-DEVD
-
 ?
BID + H2O 37°C, pH 7.0, 10 mM dithiothreitol Homo sapiens ?
-
 ?
catalytically inactive pro-caspase-2 + H2O 37°C, pH 7.0, 10 mM dithiothreitol Homo sapiens caspase-2
-
 ?
additional information PARP is not cleaved Homo sapiens ?
-
 ?
pro-caspase-7 + H2O 37°C, pH 7.0, 10 mM dithiothreitol Homo sapiens caspase-7
-
 ?
Z-VDVAD-7-amino-4-trifluoromethyl coumarin + H2O 37°C, pH 7.0, 10 mM dithiothreitol Homo sapiens 7-amino-4-trifluoromethyl coumarin + Z-VDVAD
-
 ?

Subunits

Subunits Comment Organism
dimer acive form, SDS-PAGE Homo sapiens
monomer inactive form, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
caspase-2
-
 Homo sapiens